DNA Flashcards
What is a polymer
long chains made from small repeating subunits called monomers
What are DNA and RNA types of
nucleic acids
Draw ONE DNA nucleotide
pentose sugar should be deoxyribose
nitrogenous base any except t uracil
DRAW ONE RNA NUCLEOTIDE
pentose sugar should be ribose
nitrogenous base any except thymine
Sugar - phosphate backbone is
the connection bwtn all the pentose sugars and phosphate groups
5 types of nitrogenous bses
ADENINE A
GUANINE G
THYMINE T (only in DNA)
URACIL U (only in RNA)
How many bonds does Guanine and Cytosine have?
3 hydrogen bonds
How many bonds does adenine and thymine have?
2 hydrogen bonds
are hydrogen bonds weak to break
yes they are so it’s easy in the process of DNA replication
Complete the DNA sequence
TCA ATA CGC CAC AAC CCT
AGT TAT GCG GTG TTG GGA
Calculate bases
If a DNA molecule was found to contain 15% thymine, what percentage would be cytosine?
15 T = 15 A
100-30 = 70
35% cytosine
What are the comparisons between DNA and RNA
sentences w whereas
DNA double stranded
RNA single stranded
DNA - nitrogenous base thymine
RNA - nitrogenous base uracil
DNA - pentose sugar deoxyribose
RNA - pentose sugar ribose
Draw a DNA molecule with 4 bases - CTGA
page 26
Draw a RNA molecule with 4 bases - CUGA
page 27
What about the structure of DNA will help replication??
Base paring - easy to copy strands at a time
Weak Hydrogen bonds - easy to separate to strands
Nucleotides - individual
Draw a DNA replication diagram
page 47
What are the two enzymes in DNA REPLICATION
AND THIER FUNCTIONS
DNA HELICASE- unwinds and unzips the double helix by breaking the hydrogen bonds between complimentary bases
DNA PLOYMERASE - catalyses’ the nucleotides to bond to the complimentary base and form to a new DNA strand
Where do free nucleotides come from?
they are delivered to the cell via the bloodstream
Draw a semi - conservative diagram
page 49
What is the theory of semi- conservative theory
one of the strands in each of the 2 new double helices of DNA is the original copy
What does mRNA stand for
messenger of RNA
Difference between DNA replication and transcription
DNA REPLICATION has a pentose suage of deoxyribose and a nitrogenous base of thymine whereas TRANSCRIPTION has a pentose sugar of ribose and a nitrogenous base of uracil.
What is added in the RNA PROCESSING
3’ poly-A-tail
5’ methyl cap
removing of introns
What is the FINAL product of RNA processing?
pre-mRNA
Why are the 3’ poly-A-tail and 5’ methyl cap added?
to prevent enzyme degradation in the cytosol
Does a Intron code for protein?
IT DOES NOT CODE FOR A PROTEIN
Does a Exons code for proteins?
YES IT DOES CODE FOR A PROTEIN
What do we et rid of in RNA PROCESSING
a- exons
b- introns
B
What is splicing in RNA processing?
is a process occurring during RNA processing with the removal of introns and combining of exons in a pre-mRNA strand
TRANSCRIPTION DEFINITION
DNA template strand is copied into pre-mRNA via complementary base pairing using RNA polymerase.
Pre mRNA undergoes RNA processing. Introns are removed, a 5’ methyl cap and 3’ poly-A-tail are added to form mRNA
mRNA leaves the nucleus and goes to a ribosome
What resuts in the end of DNA replication
DNA
What results in the end of TRANSCTIPTION
Pre-mRNA
What results in the end of RNA PROCESSING?
mRNA
Function of DNA is
code for proteins
What is a condon?
a sequence of 3 nucleotides in a mRNA
What is the 3 nucleotides called in DNA
triplet
What does each Condon do»???
codes for a specific and complimentary amino acid