Dna Flashcards
1
Q
Inhibitor of RNA polymerase
A
Rifampicin
2
Q
RNA primer made by this RNA polymerase
A
RNA primase
3
Q
Helicase
A
Unwinds DNA - open double helix
4
Q
DNAP III
A
adds Nucleotides to 3’ end of RNA primer (=primer extension)
5
Q
Nick translation
A
DNAP I takes over from DNAP III to remove RNA primer + replace it with new dna on lagging strand
6
Q
Seals nicks between pieces of DNA
A
DNA ligase
7
Q
Topoisomerase
A
Nicks DNA to relieve tension from unwinding
8
Q
Eukaryotes - origins
A
Eukaryotes have many origins =
9
Q
Origins that attract helicase + other replication enzymes
A
AT-rich origins
10
Q
Hayflick limit
A
Cells can only divide so many times
11
Q
FredSanger
A
‘Sequencing by synthesis’
Dna sequencing -> developed di-deoxynucleotides to terminate chain synthesis (absent hydroxyl group on 3’)
