diversity of cells and their functions Flashcards
histological techniques
a chemical that cross links proteins such as formalin is used to preserve the tissue
tissue has to be thinly sliced to preserve the tissue
Dyes
the most common dye used is a combination of haematoxylin and eosin.
haematoxylin
a basic dye that has an affinity for acidic molecules and stains them purple/blue- for example DNA or the nucleus
eosin
an acidic dye that has affinity for basic molecules and stains them pinkish red. most proteins in the cytoplasm are basic and as a result the cytoplasm is often stained pink or red.
tissue types
there are 4 basic tissue types- epithelial, connective, muscle and nervous
epithelium
cover the surface of the body, line hollow organs and also form glands. they occur as sheets of cells and vary widely in shape, size, orientation and function
connective
forms the framework of the body, but beyond that it has a dynamic role in the development, growth and homeostasis of tissues, and via fat, in energy storage
muscle
specialised to generate force by contracting
nervous
nervous tissue consists of neurons and their supporting cells. nervous tissue serves a control function and allows for rapid communication between different parts of the body
epithelia (in detail)
adhesion between epithelia cells is strong, thus forming sheets of cells. this is good for lining body cavities.
all have a layer of extracellular matrix components called basel lamina (basement membrane) to which cells are attached
all are non-vascularised
functions of epithelia
mechanical barrier (skin) chemical barrier (stomach lining) absoption (lining of intestine) secretion (salivary gland) containment (lining of urinary bladder)
classification of epithelia
shape- squamous (flattened like a fish scale)
- cuboidal (cube shaped) - columnar (like a column)
number of layers- simple (one layer)
- stratified (2 or more layers) - pseudostratified ( appears to have multiple layers but all tissue is in contact with the basal lamina)
epithelial glands
endochrine glands- product secreted towards the basal end of the cell, then distributed by the vascular system
exochrine glands- product secreted towards the apical end of the cell either into the lumen of an internal space or body surface
connective tissue
soft connective tissue- tendons, ligaments, mesentary, stroma of organs dermis of the skin etc.
hard connective tissue- bone and cartaledge
blood and lymph- specialised form of connective tissue
connective tissue- extracellular matrix
consists of- fibres: collagen, reticular and elastic fibres
- ground substance: GAGs, most of which are bound to protein cores to form glycoproteins - tissue fluid
cells
Fibroblasts: widely distributed cells that produce and maintain the extracellular matrix
adipose cells: fat cells
osteocytes: cells of bone
Chondrocytes: Cells of cartilidge
cartilage
strong, flexible, compressible, semi rigid tissue
avascular
three types, defined by the extracellular matrix: hyaline, elastic and fibrocartilage
bone
an outer shell of dense cortical bone makes up the shaft
cancellous or trabecular bone occupies the end of the bone
muscle
3 main types: smooth, cardiac and skeletal
smooth muscle
called smooth because it has no visible striations
involuntary because it isnt under conscious control and visceral because its predominantly found in organs
skeletal muscle
constitutes the muscle of the body that respond to conscious control
multinucleated, cylindrical cell
cardiac muscle
forms the major part of the walls of the heart chambers and origins of the great vessels
fibres are much shorter than striated muscle
nervous tissue
consists of neurons and support cells (glia)
surrounded by connective tissue coat- meninges for central nervous system and epineurium for peripheral nervous system
principle glia of the CNS
astrocytes: support, ion transfer, induce brain blood barrier
Oligondenrocytes: produce mylin in the brain and spinal chord
microglia: provide immune surveillence
principle glia of the PNS
schwann cells: produce myelin and support axons