digestion of carbs, proteins, lipids- overview

Question 1

what drives the absorption process?

Answer 1

Na+/K+ ATPase

Question 2

what is the only sugar not dependent on ATPase?

Answer 2

fructose

Question 3

what sugar is absorbed via secondary active transport?

Answer 3

glucose

Question 4

what is the effect of insulin on absorption in the small intestine?

Answer 4

None.

Insulin does not affect secondary active transport!
Insulin does not affect glucose transport in:
-stomach, kidney,
Insulin mainly affects in adipose tissue, resting muscles

Question 5

5 patterns of digestion and absorption

Answer 5

1. Glucose-does not require digestion
2. Protein-digested in lumen

3.Sucrose-digested by brush border
enzymes before absorption

4.Oligopeptide-directly absorbed by
cell then broken down inside cell

5.TAG-broken down before absorption
cell then resynthesize original
molecule

Question 6

small intestine overview and secretion

Answer 6

All chemical digestion & nutrients absorption occurs here
Circular folds of mucosa, villi, and microvilli – enhance s/a for absorption
Largest folds –circular folds-mucosa & submucosa-plicae circularis

Secretion
Juice secreted by small intestine does not contain any digestive enzymes
Synthesized enzymes act within brush-border membrane of epithelial cells
Enterokinase
Disaccharidases
Aminopeptidases

Question 7

Enzymes of/ into small intestine

Answer 7

Peptidase – breaks down peptides into amino acids
Sucrase, maltase, lactase – break down disaccharides into
monosaccharides
Lipase – breaks down fats into fatty acids and glycerol
Enterokinase – converts trypsinogen to trypsin
Somatostatin – hormone that inhibits acid secretion by stomach
Cholescystokinin – hormone that inhibits gastric glands,
stimulates pancreas to release enzymes in pancreatic juice,
stimulates gallbladder to release bile
Secretin – stimulates pancreas to release bicarbonate ions in
pancreatic juice

Question 8

Small intestine absorptions

Answer 8

80 % ingested water
Electrolytes
Vitamins
Minerals
Carbonates
  - active/facilitated transport
  - monosaccharides
Proteins
       - di-/tripeptides
        - amino acids
Lipids
       - monoglycerides
        - fatty acids
        - micelles
        - chylomicrons

Question 9

What happens to lactose?

Answer 9

Beta Galactosidase, also known as lactase or galactocerebrosidase, turns it into galactose (which becomes glucose) and ceramide.

(brush border enzyme)

Question 10

What happens to sucrose

Answer 10

scurase/ isomaltase converts it to fructose and glucose

brush border enzyme

Question 11

What happense to glucosylceramide?

Answer 11

beta glucosidase converts it to ceramide. (brush border enzyme)

Question 12

Overview of carb digestion and absorption

Answer 12

1. Polysaccharides into disaccharides by salivary and pancreatic amylase
2. Disaccharides into monosaccarides by disaccharidases in brush borders of S.I. epithelial cells (maltase, lactase and sucrase)
3. Glucose/galactose absorbed by Na+ and energy dependent secondary active transport
4. Fructose absorbed by passive facilitated diffusion

Question 13

2 steps of carb digestion

Answer 13

1. Intraluminal hydrolysis of starch to oligosaccharides by amylases (salivary & pancreatic)
2. Membrane digestion of oligo to mono by brush border disaccharidases (94: lactase, glucoamylase = maltase, sucrase, isomaltase)

Question 14

2 steps of carb absorption

Answer 14

1. Uptake across apical membrane into epithelial cell and

2. coordinated exit across basolateral membrane

Question 15

Protein digestion and absorption (overview)

Answer 15

Digestion begins in the stomach when pepsin digests proteins to
form polypeptides

In the duodenum and jejunum:
     - Endopeptidases cleave peptide bonds in the interior of the polypeptide:
Trypsin 
Chymotrypsin
Elastase  

 - Exopeptidases cleave peptide bonds from the ends of the polypeptide: Carboxypeptidases Aminopeptidase

Question 16

What continues protein digestion in the small intestine?

Answer 16

trypsin and chymotripsin

Question 17

What enzymes complete protein digestion in the small intestine?

Answer 17

brush border enzymes:
carboxypeptidase
aminopeptidase
dipeptidase

Question 18

How are amino acids and polypeptides transported across basolateral membrane of enterocytes?

Answer 18

Amino acids and remaining polypeptides are transported across basolateral membrane of enterocytes by facilated or simple diffusion

Question 19

Protein digestion (general)

Answer 19

1. Proteins hydrolyzed into amino acid and peptides by gastric and pancreatic proteolytic enzymes
2. Amino acids absorbed by Na+ and energy dependent secondary active transport.
   Various amino acids are transported by specific carriers
3. Small peptides are broken down into amino acids by aminopeptidases in the epithelial cells’s brush borders or by intracellular peptidases

Question 20

Can whole proteins be absorbed?

Answer 20

Enterocytes and M cells can
absorb whole proteins

Lysosomal proteases in
enterocytes degrade proteins

Question 21

H+/ oligopeptide cotransporter PepT1

Answer 21

H+/oligopeptide cotransporter PepT1 moves dipeptides, tripeptides and tetrapeptides into enterocytes across apical membrane

Peptidases hydrolyzes oligopeptides into AA

AA exit across basolateral membrane through Na+ independent AA transporters

Question 22

Harnup disease

Answer 22

Hartnup disease is an autosomal recessive disorder caused by impaired neutral (ie, mono-amino-monocarboxylic) amino acid transport in the apical brush border membrane of small intestine and proximal tubule of kidney.
Patients present with pellagra like skin eruptions, cerebellar ataxia, and gross aminoaciduria.
Hartnup disease manifests during infancy with variable clinical presentation: failure to thrive, photosensitivity, intermittent ataxia, nystagmus and tremor.
A high-protein diet can overcome the deficient transport of neutral amino acids in most patients.
Poor nutrition leads to more frequent and more severe attacks of the disease, which is otherwise asymptomatic

Question 23

Hartnup disease and the PEPT1 transporter

Answer 23

The human peptide transporter 1, PEPT1 is primarily responsible for the absorption of dietary di- and tripeptides from the small intestinal lumen.
The physiological role of PEPT1 is the absorption of small peptides from the intestinal tract after their release by enzymatic breakdown of dietary or endogenous proteins.
Thus, PEPT1 also plays a major role in the nitrogen supply to the body.
Presence of PepT1, individual do not become deficient in amino acids
A high-protein diet can overcome the deficient transport of neutral amino acids in most patients

Question 24

Where do fats go?

Answer 24

fatty acids and monoglicerides enter the lacteals of hte villi and are transported to the systemic circulation via the lymph in the thoracic duct (glycerol and short-chain fatty acids are absorbed into the capillary blood in the villi and transported to the liver via the hepatic portal vein)

Question 25

Digestion and absorption of lipids

Answer 25

Arrival of lipids in the duodenum serves as a stimulus for secretion of bile Emulsification: - bile salts micelles are secreted into duodenum to break up fat droplets Pancreatic lipase and colipase hydrolyze triglycerides to free fatty acids and monoglycerides - colipase coats the emulsification droplets and anchors the lipase enzyme to them - forms micelles and move to brush border Free fatty acids, monoglycerides and lysolecithin leave micelles and enter into epithelial cells - resynthesize triglycerides and phospholipids within cell combine with a protein to form chylomicrons Secreted into central lacteals

Question 26

Another card on the digestion and absorption of lipids

Answer 26

Fat is not soluble in water It undergoes a series of transformations for digestion and absorbtion ``` 1. Dietary fat (TGs) emulsified by bile salts into fat droplets 2. Lipase hydrolyses TGs into mono-glycerides & free FAs 3. These water insoluble products are carried in interior of water- soluble micelles to luminal surface of S.I. epithelial cells 4. Micelle in epithelial surface, mono-glycerides & free FAs leave micelles & passively diffuse through luminal membranes 5. Mono-glycerides & free FAs resynthesized into TGs inside epithelial cells 6. TGs aggregate & coated with lipoprotein to form water soluble chylomicrons 7. Chylomicrons enters lacteals ```

Question 27

Transport and aborption of lipids

Answer 27

In blood, lipoprotein lipase hydrolyzes triglycerides to free fatty acids and glycerol for use in cells Remnants containing cholesterol are taken to the liver: - form VLDLs which take glycerides to cells - once glycerides are removed, VLDLs are converted to LDLs LDLs transport cholesterol to organs and blood vessels HDLs transport excess cholesterol back to liver

Question 28

Large intestine

Answer 28

The main functions of the large intestine are: - completion of fluid absorption most water absorption occurs in the right colon (the cecum, the ascending colon and the first half of the transverse colon) - active secretion active secretion of K+ and HCO3- into colonic lumen ``` - absorption Na+ actively absorbed bile salts vitamins produces by bacteria-organic molecules cofactors or coenzymes ``` - storage and elimination of fecal waste prior to defecation, fecal waste is stored in left colon (distal half of the transverse colon, descending colon and sigmoid colon) by the time fecal material reaches the rectum, it consists of a small volume of K+ rich fluid containing undigested plant fibers, bacteria, and inorganic material.