Digestion And Absorption Flashcards

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1
Q

What happens during digestion

A

Large biological molecules are hydrolysed to smaller molecules that can be absorbed across the cell membrane

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2
Q

Test for starch

A

Iodine
Positive- blue-black
Negative - orange- brown

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3
Q

Test for sugars

A

Benedict’s
Positive- brick-red
Negative - light blue

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4
Q

Test for proteins

A

Biuret
Positive- lilac -purple
Negative blue

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5
Q

Function of exopeptidase

A

Hydrolyse peptide bonds at end of polypeptide

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6
Q

Function of endopeptidase

A

Hydrolyse internal peptide bonds within polypeptide

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7
Q

Explain the digestion of lipids with in gut (exclude absorption)

A

Triglycerides decrease because of the action of lipase. They also decrease because of they are hydrolysed into fatty acids which causes the concentration of fatty acids to increases. They also decrease because of the digestion of the ester bonds.

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8
Q

Describe the role of micelles in the absorption of fats into the cells lining the ileum.

A

Micelles include bile salts and fatty acids
They make fatty acids more soluble in water
They bring the fatty acids to the lining of the ilium and releases it into cell

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9
Q

In calorimeter the higher the absorbency the darker/lighter the colour of the mixture

A

Darker the mixture
( it’s the absorbency of the substance rather than the absorbable on the other side)

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10
Q

A student did not have a colorimeter describe how a method this student could use to prepare colour standards and use them to give data for the total anthoyancic extracted

A

Use known concentration of pigment
Prepare dilution series
Compare results with colour standards to give concentration

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11
Q

What does bile do to fats

A

Emulsification

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12
Q

Where is amylase produced

A

Salivary glands
Pancreases
Small intestine

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13
Q

How does amylase work

A

It hydrolyses the polysaccharide into disaccharide by hydrolysing the glycosidic bond

not into monosaccharides because then the specific enzyme does it E,g. Maltase then digests monosaccharides maltose)

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14
Q

Enzyme that break down
Sucrose
Lactose
Maltose

A

Sucrase
Lactase
Maltase

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15
Q

Where does protein digestion start

A

Stomach

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16
Q

What are lipids digested by 2

A

Bile salts
Lipase

17
Q

Where is lipase produced

A

Pancreas

18
Q

How does lipase work

A

Hydrolyses the ester bond in triglycerides to for glycerol and fatty acids

19
Q

Where are bile salts made

A

Liver

20
Q

How do bile salts work + why is this advantage

A

The work in the small intestine
Provides a larger surface area for lipas etc act on so faster hydrolysis of lipids. Breaks ester bonds

21
Q

Which is physical and which is chemical in lipid digestion

A

Bile salts - physical
Lipase - chemical

22
Q

Where are bile salts stored

A

Gall bladder

23
Q

What are micelles made of

A

Fatty acids
glycerol
Monoglycerides
Bile salts

24
Q

What happens when the micelles have released lipid into cell

A

Reform into triglycerides They are combined with proteins in the Golgi apparatus and form chylomicrons to large to diffuse into blood stream so are released into the lacteal

25
Q

How is a chylomicron formed

A

Addition of a protein onto a triglycerides

26
Q

On which biological molecule do endo and exo peptides work

A

Proteins

27
Q

What does the dipeptidase enzyme do

A

Hydrolyses dipeptides into amino acids by breaking ester bond

28
Q

Which system is lacteal part of

A

Lymph

29
Q

Explain how starch is digested

A

Starch broken down by amylase into disaccharides ( maltose, sucrose lactose)
The disaccharides are then broken down by membrane-bound disaccharides (sucrase, lactase, maltase)

30
Q

Role of bile salts

A

Emulsification of lipids to form micelles to create larger surface area for lipase to work on

31
Q

Explain the journey of the micelles

A

1) micelles releases fatty acids and monoglycerides into the ilium
2) the endoplasmic reticulum reforms them into triglycerides
3)the Golgi adds a protein to form chylomicron
4) transported to lacteal via exocytosis

32
Q

Advantage of lipid droplets and micelle formation x3

A

Droplets increase surface area for lipase
So after hydrolysis of lipids
Micelles carry fatty acids and glycerol through the membrane to the cell

33
Q

Role of Golgi apparatus in absorption of lipids

A

Processes triglycerides
Combines triglyceride with protein to form chylomicron
Packages for releases