Diagnostics Flashcards
What ketones are detectable on urine sticks?
Acetone and acetoacetic acid not bhb
Bhb
Beta hydroxy butyrate
Ifa
Serum incubated with slides containing immobilised antigen
Secondary antibody labelled with fluorescent marker
Conventional pcr
Series of heated and cooled cycles using T dependent dna polymerase and code specific primers used to amplify small quantities of dna
Nested pcr
2 sets of primers
Second primer is directed against a segment of codes from first product
= Reduced amplification of inappropriate dna
qRT-PCR
Real time pcr
After every cycle amounts are measured in fluorescent units.
Quantities measurement of dna in original sample
RT-PCR
Reverse transcriptase pcr
Reverse transcriptase is added to produce cDNA from RNA
Rest as qRT-PCR
Aqueocenthesis
Collect AH + sample masses Under heavy sedation or GA Pediatric tubes Culture- plain , cytology edta 0.1- 0.2 ml
Ah analysis
No cells or culture
TP 21-37mg/dl
Neg serology
True positive and true negative test
T+ = + test with an animal which has the disease
T- = - test with an animal which does not habe the disease
Sensitivity
True positive/
animals with disease (t+and f-)
×100
Highly sensitive test
Good screening test
Almost always detects disease
Specificity
True negatives /
Animals without disease (t- and f+)
X 100
Highly specific test is unlikely to give false + so positive result should be diagnostic
Diagnostics accuracy
Number correctly classified / number animals in the study ×100
PV of positive and negative test
Predictive value of positive test
(Probability of + test to be correct)
(T+/all +)x100
Pv of negative test
(T-/all -)x100
