Diagnostic Tests

Question 1

Western Blot

Answer 1

Detects target level proteins

Used to confirm HIV diagnosis

Question 2

Sandwich ELISA

Answer 2

Measures Antigen
Used for MI diagnosis (Troponin T and I)
Used for pregnancy tests (hCG)

Question 3

Indirect ELISA

Answer 3

Measures Antibody

Used for HIV diagnosis

Question 4

Restriction Fragment Length Polymorphism

Answer 4

“DNA Fingerprinting”

Used for forensics, paternity tests and disease detection

Question 5

Variable Number Tandem Repeats

Answer 5

Used to identify severity of diseases (Huntington’s)

Question 6

Southern Blotting

Answer 6

Test for DNA

DNA-DNA hybridization

Question 7

Northern blotting

Answer 7

Test for RNA

DNA-RNA hybridization

Question 8

qPCR

Answer 8

Used to quantify gene expression and number of infectious agent genes