Diagnostic and Typing Methods Flashcards

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1
Q

what bacteria are associated with periodontal disease

A

porphyromonas gingivalis
prevotella intermedia

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2
Q

what bacteria is associated with dental caries

A

streptococcus mutans

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3
Q

what bacteria is associated with root canal infections

A

porphyromonas endodontalis
fusobacterium nucleatum

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4
Q

how would you do a microbial culture

A

culture on suitable agar medium
isolate bacteria
identify by characterisation of enzyme activities, sugar fermentation

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5
Q

what molecular biological methods are available for bacterial detection

A

DNA probes
PCR

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6
Q

what are the advantages of using culture methods

A

yields bacterial isolates for future testing and study

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7
Q

what are the disadvantages of culture methods

A

requires viable cells
insensitive
only small numbers of samples can be analysed at once
inconclusive results
labour intensive

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8
Q

what are the types of DNA probe

A

whole genomic
cloned gene

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9
Q

what is PCR used for

A

directly identifying bacteria in clinical specimens

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10
Q

what gene do PCR primers target

A

16S rRNA gene

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11
Q

what are the advantages of PCR and DNA probes

A

less time consuming than culture methods
very sensitive
directly detect bacterial DNA within clinical samples
does not require viable cells

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12
Q

what are the disadvantages of PCR and DNA probes

A

may detect dead cells
detects only pre-selected species

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13
Q

why is it important to subtype bacteria

A

track routes of transmission during disease outbreaks
study pathogenicity of specific strains

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14
Q

what are the molecular typing methods

A

restriction enzyme analysis
gene probe typing
ribotyping
16S-23S intergenic spacer region
DNA sequencing

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