diagnosis of infection Flashcards
1
Q
What are 3 common types of agar used?
A
- blood
- chocolate
- CLED agar
2
Q
What is typical of E.coli on a CLED agar?
A
produces yellow colour as bacteria ferments lactose to produce acid
3
Q
What are the 3 lab techniques?
A
- microscopy
- culture (nutrients and agar plates)
- difficult-culture organisms
4
Q
What are the lab techniques for difficult-to-culture organisms?
A
- serological techniques
- molecular techniques (PCR)
- tissue culture
5
Q
What does Neisseria Meningitidis look like under the microscope?
A
gram negative diplocci
6
Q
In serology, what is being looked for?
A
antibodies
- IgM for acute infection
- IgG for long standing immune response
7
Q
What is serology used for?
A
- viral infections
- chlamydia and mycoplasma
8
Q
What are some examples of latency
A
- cold sores (HSV)
- tuberculosis
-chicken pox reactivating as Herpes zoster (shingles) - CMV and EBV
9
Q
What is viral encephilitis?
A
- viral infection of brain tissue
- fever, headache, confusion, fits
10
Q
What are causes of viral encephilitis?
A
- enteroviruses most common
- herpes viruses (HSV, VZV, CMZ, EBV)
11
Q
What organisms is tissue culture used for?
A
- viruses
- mycoplasma
- chlamydia
12
Q
What are the two ways of detecting organisms in tissue cultures?
A
- cytopathic effect on cells
- expression of viral proteins detected at the cell surface
