Detmine Vmax and Km Flashcards
1
Q
What is Vmax?
A
The maximum velocity of reaction(Top of the curved-line)
2
Q
What is the rate of 1/2 Vmax?
A
It is the Vmax times 1/2.
3
Q
What is Km?
A
The concentration of substrate at which the reaction occurs at the rate of 1/2 Vmax.
Loc: find the 1/2 Vmax and then find it’s x-axis value.
4
Q
Which is better Lineweaver-Burke Plot or Eddie-Hostee?
A
Eadie-Hofstee
P.S. Lineweaver-Burke Plot with small experimental error can result in large error in the graphically determined values.
