Determining water potential by mass change Flashcards
potential control variables of potato experiment
temperature, type of potato
Iv of experiment
concentration of NaCl
DV of experiment
% change of mass
Describe the set up of the test tubes
-6 boiling tubes labelled 0, 0.2, 0.4, 0.6, 0.8 and 1.0 mol dm-3
-place 30cm3 of distilled water in first tube
-place 30cm3 of appropriate salt solution in other tubes
-firmly stopper each tube
Describe procedure
-use cork borer and scalpel to prepare 6 potato cylinders of 50mm long
-divide filter paper into 6 labelled sections and place cylinder onto each section
-record mass of each cylinder and place in appropriate test tube
-leave tubes for 45 mins
-remove cylinders and place on appropriate space
-blot each cylinder dry
-reweigh each cylinder and calculate mass change
What results would you expect to see?
As NaCl concentration increases, potatoes lose mass more rapidly
what happens when the potato reaches its isotonic point?
the movement of water into the cells is equal to the movement of water out of the cells
Explain why the graph would show a negative correlation
-when the NaCl solution has a higher solute concentration than the potato, water moves out of the potato so it therefore loses mass