Denaturation And Renaturation

Question 1

What effect does heat and radiation have?

Answer 1

Increased kinetic energy can make atoms vibrate. Breaks hydrogen and ionic bonds.

Question 2

What effect do strong acids and alkalis have?

Answer 2

pH alters charges on R groups.

Ionic bonds disrupted. Prolonged exposure can lead to peptide bonds being broken.

Question 3

What effect do heavy metal ions have?

Answer 3

Heavy metal actions from bonds with negatively charged R groups. Disrupts hydrogen and ionic bonds.
Reduce overall charge on a protein lowering solubility.

Question 4

What effect do organic solvents and detergents have?

Answer 4

Hydrophobic groups will dissolve in organic solvents. Protein opens up to expose its hydrocarbon R groups

Question 5

How is a protein denatured?

Answer 5

Bonds are altered changing its shape. 
Heat/ radiation
Strong acid/ alkali
Heavy Metal ions 
Organic solvents and detergents

Question 6

How can renaturaton occur?

Answer 6

Only after likited denaturstion the protein may be able to regain its shape