Definitions Flashcards
Define metabolism
Transformation of one chemical to another thru series of enzyme controlled reactions. Broken down by respiration for energy or synthesis into vital substances
imprinting
Parental specific gene expression in diploid cells
Validation
Confirmation using evidence that requirements for intended use(test) have been met. Where novel test/tech. To define performance spec of test.
ongoing process of monitoring a test, procedure, or method to ensure that it continuously performs as expected …“Does the test still work?
Verification
Compare test performance characteristics to perf spec. Use when CE marked test or new test in existing technique eg new gene panel.
…one-time process performed to determine or to confirm a test’s expected performance prior to implementation in the clinical laboratory…“Does the test work?”
Sensitivity
Proportion positive results correctly ID by test. True positive/ true pos + false neg X 100
Specificity
Proportion negative results correctly ID by test
True negative/ true neg + false pos X 100
Accuracy
How often test gives correct result
True result/ true results + false results X 100
Trueness
How dose test result is to reference value
Precision
Degree to which separate measurements differ which indicates how well a single test result is representative of a number of repeats
Repeatability
Closeness of results obtained when using same samples under same test conditions. Within run precision
Reproducibility
Closeness of results obtained when using same samples under DIFFERENT test conditions. Eg technicians Between run precision
Robustness
Reliability of test. How well maintain precision when certain variables changed eg sample quality
Limit of detection
Lowest quantity of an analyse that can be reliably detected in a test
Limit of quantification
Extremity at which accurate quantification can still be achieved
Accreditation
The assessment of an organisation to ensure it is performing in accordance with the requirements of a required standard or regulation
