Deck 9 (slides 45-52)

Question 1

The Human Genome project was done using “_________ sequencing.”

Answer 1

Shotgun

Question 2

First, cut DNA up into random _______.

Answer 2

Fragments

Question 3

Then, determine the base sequence of each fragment using DNA sequencing (with the _______ bases).

Answer 3

Dyed

Question 4

Then use a computer to find areas of ________ between the fragments.

Answer 4

Overlap

Question 5

Then put the fragments together by _______ overlapping areas.

Answer 5

Linking

Question 6

Scientists were surprised to find that only a small part of a human DNA molecule was made up of _______ (___%).

Answer 6

Genes, 2

Question 7

They were also surprised how few genes humans had (_________).

Answer 7

20,000

Question 8

Researchers around the world are ________ the huge amount of info in the DNA sequence; looking for genes that may provide useful clues to some of the basic properties of ______.

Answer 8

Analyzing, life

Question 9

Biotech companies are rushing to find genetic info that may be useful in developing new drugs and treatments for ______; like _______ _______.

Answer 9

Disease, gene, therapy

Question 10

Gene therapy is a possible way of ________ genetic disorders.

Answer 10

Curing

Question 11

Gene therapy is the process of ________ the gene that causes the genetic defect.

Answer 11

Changing

Question 12

The body can then make the _________ protein or enzyme it needs, which ________ the disorder.

Answer 12

Correct, eliminate

Question 13

In _______, a young French girl was apparently cured of an inherited immune disorder.

Answer 13

1999

Question 14

Cells from her bone _______ were removed and modified in the lab (the ________ gene was put into them); the cells were then _______ back into her body.

Answer 14

Marrow, good, placed

Question 15

________ are often used in gene therapy because of their ability to ________ DNA into cells.

Answer 15

Viruses, inject

Question 16

The virus is __________ so that they cannot cause disease.

Answer 16

Modified

Question 17

The replacement gene is ________ into the virus’ DNA.

Answer 17

Spliced

Question 18

The patient is then _________ with the modified virus, which should carry the gene into cells to ________ genetic defects.

Answer 18

Infected, correct

Question 19

Gene therapy experiments have not always been _________.

Answer 19

Successful

Question 20

Attempts to treat CF by ________ genetically engineered viruses into breathing passages have not produced a lasting ________, although gene therapy still holds great promise.

Answer 20

Spraying, cure