Deck 4 Flashcards

1
Q

in which test do we need to find a precipitation line

A

AGID test

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2
Q

Cyclozoonoses require:

a) One vertebrate host
b) More than one vertebrate host
c) Vertebrate and invertebrate host

A

More than one vertebrate host

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3
Q

immunofluorescence detects

A

Ag

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4
Q

wavelength for immunofluorescence/from immunofluoroscope

A

490-630nm, emission peak is 515nm

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5
Q

haemagglutination test (HIT) detects

A

viral Ab

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6
Q

PCR procedure

A

denaturation, annealing, extension

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7
Q

what is real time PCR

A

PCR with real time result after each cycle

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8
Q

annealing temperature of PCR

A

40-60 celcius (52 is ideal)

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9
Q

skin contamination is cleaned by

A

0.5% chloramine B or septonex 1%

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10
Q

4 stages of phagocytosis

A

chemotaxis, adherence, ingestion, digestion

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11
Q

excision of histological sample

A

1cm maximum

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12
Q

blastotransformation is

A

a method to determine the function and state of immune system in a certain organism by stimulating the proliferation of lymphocytes

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13
Q

enzyme most commonly used in ELISA

A

horseradish peroxidase

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14
Q

conjugation involves

A

Ig, serum and fluorochrome

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15
Q

PCR is

A

amplification of specific DNA fragments on targeted species

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16
Q

direct ELISA detects

A

Ag

17
Q

phagocytes of mammals

A

neutrophils, macrophages, monocytes

18
Q

Synthetic immunomodulators are:

a) Glucans
b) Cyclophosphamide
c) Levamisole, Isoprinosine

A

Levamisole, Isoprinosine

19
Q

ELISA test is for detection of Ab or Ag

A

sandwich and competitive ELISA detect Ag
indirect and blocking ELISA detect Ab

20
Q

name the serological methods

A

agglutination methods (rapid slide agglutination, commercial agglutination, tube agglutination)
complement fixation test
virus neutralisation test
immune diffusion test
haemagglutination test
ELISA test