Deck 4 Flashcards
in which test do we need to find a precipitation line
AGID test
Cyclozoonoses require:
a) One vertebrate host
b) More than one vertebrate host
c) Vertebrate and invertebrate host
More than one vertebrate host
immunofluorescence detects
Ag
wavelength for immunofluorescence/from immunofluoroscope
490-630nm, emission peak is 515nm
haemagglutination test (HIT) detects
viral Ab
PCR procedure
denaturation, annealing, extension
what is real time PCR
PCR with real time result after each cycle
annealing temperature of PCR
40-60 celcius (52 is ideal)
skin contamination is cleaned by
0.5% chloramine B or septonex 1%
4 stages of phagocytosis
chemotaxis, adherence, ingestion, digestion
excision of histological sample
1cm maximum
blastotransformation is
a method to determine the function and state of immune system in a certain organism by stimulating the proliferation of lymphocytes
enzyme most commonly used in ELISA
horseradish peroxidase
conjugation involves
Ig, serum and fluorochrome
PCR is
amplification of specific DNA fragments on targeted species