Deck 2

Question 1

Complement fixation test is used for detection of

a) antigen
b) antibody
c) viral genome

Answer 1

antibody

Question 2

In sandwich ELISA positive result is:

a) change of colour
b) no colour

Answer 2

change of colour

Question 3

Carnivores are born

a) agammaglobulinemic
b) hypogamaglobulinemic

Answer 3

hypogamaglobulinemic

Question 4

What is tropisms of pathogens?

Answer 4

the ability of pathogen to infect a certain organ or system

Question 5

Name at least 3 tests for cellular immunity evaluation?

Answer 5

lymphocyte proliferation assay
flow cytometry
agar gel immunodiffusion test
complete blood count
ingestion
lymphocyte proliferation assay
INT test

Question 6

monotropic

Answer 6

1 organ or system

Question 7

polytropic

Answer 7

more organs or systems

Question 8

pantropic

Answer 8

multiple sites in organism

Question 9

what is the role of colostral immunity

Answer 9

to provide passive immunity and protection against septicaemia and organ infection, as well as local protection of intestne and GIT bacteria multiplication

Question 10

Clinical signs indicating failure of passive transfer of immunity.

Answer 10

at age 3-5 days: profuse diarrhoea and dehydration, respiratory or systemic infections

Question 11

29. What sample is taken for rabies diagnosis?

a) saliva
b) blood
c) brain

Answer 11

brain

Question 12

Cytopathic effect is:

a) genetic mutation of cell culture after virus infection
b) monolayer duplication after virus inoculation
c) destruction of the cells after virus inoculation

Answer 12

destruction of the cells after virus inoculation

Question 13

What are the main reagents in ELISA test:

Answer 13

detection Ag or Ab, coating buffer, washing buffer, protein stabilisers/blockers, sample

Question 14

What are advantages of real time PCR comparing with classical PCR?

Answer 14

in classical PCR, the amplified DNA product is detected in an end-point analysis
in real time PCR, the accumulation of amplification product is measured as the reaction progresses, in real time, with product quantification after each cycle and shown on a monitor

Question 15

Division of etiological agents according to their pathogenicity

Answer 15

obligatory pathogenic, facultative pathogenic, apathogenic

Question 16

Give an example of non-contagious infectious diseases:

Answer 16

rabies, lyme disease

Question 17

Describe risk assessment in epizootiology

Answer 17

pathogenicity, mode of transmission, hosts, zoonotic or not, mortality

Question 18

PCR test is used for the detection of:

a) antigen
b) antibody / antibody titre
c) genome or part of genome / etiological agent nucleic acids

Answer 18

genome or part of genome / etiological agent nucleic acids

Question 19

Division of cell cultures according to the growth potential:

a) primary and established
b) monolayers and established
c) monolayers and suspension

Answer 19

primary and established

Question 20

In haemagglutination-inhibition test positive result is:

a) inhibition of the blood cell agglutination – “button”
b) haemagglutination – diffuse layer of the cells

Answer 20

inhibition of the blood cell agglutination – “button”