De Girolamo 3

Question 1

How many ozakzaki fragments?

Answer 1

3

Question 2

What does the 1st Okazaki fragment do?

Answer 2

Made in the opposite direction of lagging strand

Question 3

How is the 2 O fragment made?

Answer 3

Leading strand elongates and the 2nd is made

Question 4

4 major stages of DNA rep?

Answer 4

1-initiation and unwinding
2-primer synthesis
3-elongation
4-termination

Question 5

How much does mis pairing occur?

Answer 5

1 in 10 to the power of 8

Question 6

High replication accuracy arises because…?

Answer 6

1-cell maintain balanced leeks of dNTPs reducing chances of mis incorporation
2-DNA polymerase detects and terminates the errors

Question 7

Point mutation?

Answer 7

1 base replaces the other

Question 8

2 types of point mutations?

Answer 8

Transitions—>purine replaces purine

Transversions–>purine replaces pyrimidine

Question 9

What’s the Mismatch repair system?

Answer 9

System that corrects errors which are missed by DNA polymerase

Question 10

Process of mismatch repair?

Answer 10

Endonucleass exercises mismatch
DNA pol 2/3 fills in gap
DNA ligase resells strand

Question 11

What’s ner?

Answer 11

Nucleotide excision repair

Question 12

How is overall 3-5’ DNA chain growth achieved?

Answer 12

Both strands polymerised 5’-3’ direction

Synthesised as one continuous molecule