De Girolamo 1

Question 1

What did Griffith do?

Answer 1

Bacterial transformation experiment

Question 2

What was the conclusion of the bacterial transformation experiment?

Answer 2

Genetic material from the heat killed S type has been transferred to living R type and provided a new trait

Question 3

What’s the interpretation of the bacterial transformation experiment?

Answer 3

Something in the virulent S strain could transform non virulent R strain into a lethal form, even when killed

Question 4

What’s DNA replication?

Answer 4

DNA unwinds to form 2 replication forks (bidirectional replication)

Question 5

What happens in initiation and unwinding?

Answer 5

1. assembly of rep. Fork
2. recruits dnaA and dnaB and DNA primase
3. helicase bound to protein (dnaC) which is released, helicase starts to unwind

Question 6

What’s primosome synthesis?

Answer 6

Protein assembly which involves helicase and primase
Primase adds the nucleotides
Produces RNA segment

Question 7

What does DNA helicase do?

Answer 7

Break H bonds

Question 8

SSB protein?

Answer 8

Single stranded binding protein

Function:stop the unwinding of DNA from rejoining

Question 9

Topoisomerase?

DNA gyrase

Answer 9

Reduce the torsional strain caused by the unwinding of the double helix

Question 10

What did chargaff suggest?

Answer 10

That DNA was made up of equal quantities of purines and pyrimidines

Question 11

What did rosalin Franklin suggest?

Answer 11

X Ray differentiation suggested there was a helical structure

Question 12

4 required properties

Answer 12

VIRT
Variation-phenotype variation 
Infomation-must contain information necessary to construct an entire organism
Replication
Transmission