Day 42

Question 1

The Chemiosmotic Theory

Answer 1

1) oxidative phosphorylation requires intact inner mitochondrial membranes
2) Inner membrane is impermeable to ions
3) Electron transport results in H+ transport out of mitochondria
4) Compounds that increase inner membrane permeability to protons allow electron transport to occur, but inhibit ATP synthesis. Known as uncoupling.

Question 2

Chemiosmotic Coupling

Answer 2

Chemical - [H+] gradient across inner membrane

electrical - membrane potential across inner membrane generates proton motive force (pmf) to drive atp sythesis

Question 3

Proton Motive Force

Answer 3

1) membrane potential aids in transport of protons across inner membrane. the inside is more negative that intermembrane space. Good because more negative from e- will attract H+ and help pull them right on back into the matrix
2) Concentration gradient….more in innermembrane space helps flow into matrix

Question 4

FoF1 ATP Synthase

Answer 4

• All over the inner membrane to aid in facilitated diffusion of protons and generates ATP
• Fo is a H+ channel that is connected via a stalk to F1 which is the catalytic

Question 5

F1 Component

Answer 5

Contains subunit that connects it with membrane, and alpha and beta subunits that bind and make ATP

Question 6

F0 Component

Answer 6

Contains subunits within the membrane including c subunits that form a ring of antiparallel coild coils and changes confomation when bound to proton . it shuttles.