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Hematology > Day 4 > Flashcards

Day 4 Flashcards

1
Q

What hematological determination is used to classify anemia’s based on the morphological characteristics of the RBCs?

A

RBCs Indices

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2
Q

What does the MCV tell you about the RBCs?

A

Indicates the size of the RBC

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3
Q

What does the MCH tell you about the RBCs?

A

weight(mass) of hemoglobin

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4
Q

What does the MCHC tell you about the RBCs?

A

Tells what percentage of RBCs is hemoglobin

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5
Q

What does the RDW tell you about the RBCs?

A

Degree of anisocytosis is present, or degree of variability of area cell

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6
Q

What is the unit of measure for MCV?

A

Femtoliters (fl)

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7
Q

What is the unit of measure for MCH?

A

Picograms (pg)

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8
Q

What is the unit of measure for MCHC?

A

g/dl or percentage (%)

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9
Q

What is the unit of measure for RDW?

A

Percent(%)

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10
Q

What is the calculation for MCV?

A

MCV= HCT% x 10
———————
RBC count

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11
Q

What is the calculation for MCH?

A

MCH= HGB g/dl x 10
————————-
RBC count

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12
Q

What is the calculation for MCHC?

A

MCHC= HGB(g/dL) x 100
—————————-
HCT

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13
Q

What is the calculation for RDW?

A

RDW= SD OF MCV x 100
——————-
Mean MCV

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14
Q

Which RBC indice is the most reliable automated index?

A

Mean Corpuscular Volume (MCV)

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15
Q

Which RBC indice is the most effective discriminant for classification of anemia’s?

A

Mean Corpuscular Volume (MCV)

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16
Q

Name one disease state in which one many have a MCV of 60-70 fl?

A

Iron Deficiency Anemia

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17
Q

What is the normal range for MCH?

A

27-33pg

18
Q

What does MCHC valve of 40+ indicate?

A

malfunction or error in manual calculation

19
Q

Which RBC indice is the least reliable in automated methods?

A

Mean Corpuscular Hemoglobin Concentration (MCHC)

20
Q

What is the reference range of RDW?

A

11-15%

21
Q

What blood film method is used by NCCLS as a reference method?

A

Wedge Blood Film( blood smear)

22
Q

What is the purpose of Methanol in the Wright-Giemsa stain?

A

Kills and fixes cels

23
Q

What type of dyes are used in the Wright-Giemsa stain?

A

Basic and acidic aniline dyes

24
Q

Explain the pH and staining characteristics of Wright-Giemsa stain.

A

Cells with high pH(base) stain blue(basophils)

Cells with low pH(acid) stain orange(eosinophils)

25
Q

Explain what the RBCs and Monocytes should look like Ifthe Wright-Giemsa stained properly?

A

RBCs should be pink to red-orange

Monocytes should have light purple nuclei; cytoplasm should be blue-gray

26
Q

Explain what the cells will look like if the stain is too basic.

A

Cells would be overstained, giving an overall blue effect(including RBCs); nuclear chromatin will stain dark blue to black.

27
Q

Explain what would happe if stain is too acidic.

A

RBCs too red, while WBCs and platelets appear pale.

28
Q

In the Wright-Giemsa stain, how do granules stain?

A

Bright orange in eosinophils and dark blue in basophils

29
Q

In the Wright-Giemsa stain, how do platelets stain?

A

Violet to purple

30
Q

In the Wright-Giemsa stain, how should lymphocytes stain?

A

Dark purple nuclei and cytoplasm should be clear to robins egg blue

31
Q

In the Wright-Giemsa stain, how should neutrophils stain?

A

Dark purple nuclei and cytoplasm should be light pink with lilac granules

32
Q

What is the reference range for MCV?

A

80-96 fl

33
Q

RDW measurement is derived from automated cell counters that ____________ measure the MCV.

A

Directly

34
Q

The MCHC only increases in _________________; it is decreased or remains normal in other anemia’s.

A

Spherocytosis

35
Q

What is indicated in a MCHC below 32?

A

Hypochromasia

36
Q

What is the MCHC limit for hemoglobin solubility?

A

37 g/dL

37
Q

MCH may be as high as ____ in macrocycytic anemia.

A

50pg

38
Q

MCH may be as low as ____ or les in microcytic anemias

A

20pg

39
Q

Other than MCV , what other indice is a reliable automated index?

A

MCH

40
Q

In what type of anemia may the MCV be up to 150 fl?

A

Pernicious anemia

Hematology (6 decks)

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