Data Fitting

Question 1

What are the steps of data fitting?

Answer 1

• Use the experimental data to generate an estimate for the parameters (e.g. Kd or Km), insert this into the provided mathematical function of the graph to generate a curve.
• Calculate the difference between these estimated values and the raw data values
• Calculate the sum of the chi squared values ∑(raw – estimate)2
• Use the solver function to minimise the values of the chi-squared through altering the parameter (Kd value)
• Repeat the use of this function until the Kd remains unchanged
• Now the function is no longer an estimate but a best fit for the data, the Kd value now being the best value match for the experimental data.