Dalton: PSCs and Cell Therapy

Question 1

two types of stem cells

Answer 1

embryonic and adult

Question 2

PSCs can be used as model for these events

Answer 2

early developmental events

Question 3

PSCs can be used to screen for:

Answer 3

drugs

Question 4

PSCs can be used as a model for this

Answer 4

disease

Question 5

PSCs can be used for:

Answer 5

cell therapy

Question 6

these divide indefinitely

Answer 6

ESCs

Question 7

definition: have capacity to differentiate into all cell types

Answer 7

pluripotent

Question 8

these have capacity to differentiate into all cell types

Answer 8

ESCs

Question 9

pluripotent cells from fertilized egg are isolated from blastocyst:

Answer 9

inner cell mass (ICM)

Question 10

process makes pluripotent cells become more specialized

Answer 10

differentiation

Question 11

won 2007 nobel prize for derivation of embryonic stem cells from mouse embryos

Answer 11

Martin Evans

Question 12

process where outer cells lysed and pipette releases cluster of pluripotent cells

Answer 12

immunosurgery

Question 13

person derived embryonic stem cells from human embryos

Answer 13

jamie thomson

Question 14

derivation of hESCs: after cleavage stage, embryos cultured to this stage

Answer 14

blastocyst

Question 15

derivation of hESCs: days embryos cultured to blastocyst stage

Answer 15

5 days

Question 16

derivation of hESCs: after embryos cultured, cell isolated by:

Answer 16

immunosurgery

Question 17

derivation of hESCs: after immunosurgery, cells plated on:

Answer 17

feeder layer

Question 18

structure hESCs grow as

Answer 18

sheets

Question 19

hESCs have high volume ratio of:

Answer 19

nuclear : cytoplasmic

Question 20

rate of hESC growth

Answer 20

rapid

Question 21

hESCs form these when transplanted into immuno-comprimised mice

Answer 21

teratomas

Question 22

hESCs can be stored by:

Answer 22

freezing

Question 23

mESCs can reintegrate into:

Answer 23

blastocyst embryos

Question 24

ESCs do not undergo replicative:

Answer 24

senescence (aging)

Question 25

4 types of tissue types pluripotent ES cells differentiate into:

Answer 25

ectoderm, endoderm, mesoderm, extraembryonic

Question 26

2 advantages of iPSCs over hESC

Answer 26

patient-specific, ethical issues

Question 27

disadvantages of Yamanaka’s method:(3)

Answer 27

efficiency, c-myc an oncogene, viral DNA genome modification

Question 28

two diseases modeled with iPSCs

Answer 28

Hirschsprung’s, Treacher-Collins

Question 29

three therapies with iPSCs deal with:

Answer 29

spinal cord injuries, diabetes, macular degeneration