Dairy Flashcards
What does milk contain
lipids, proteins, salts, carbohydrates and many other miscellaneous constituents
What are the essential nutrients found in milk
Vit A, Vit B12, riboflavin, calcium, potassium, magnesium, zinc, phosphorus, carbohydrate, protein
‘raw’ milk
straight from animal
where is milk made?
What is it made from?
In the mammary gland, in units called alveolus - which consists of secretory cells surrounded by blood vessels.
Lactose, fat and most proteins (casein) formed from substrates carried in the blood, other things are simply filtered from the blood.
400L-800L of blood components needed for 1L of milk
What is the composition of cow’s milk
87% water
9% non-fat solids (SNF)
4% fats
How does the composition of Cow’s milk compare to Humans?
more casein, less lactose.
What affects the composition of milk
nutritional factors (type and quality of feed) and non-nutritional factors (breed, stage of lactation, season and temperature, age, size, milking frequency, disease)
breed- holstein usually used: lower fat, lower protein, lower % solids
lactation- increase in fat and protein w time, decrease in lactose.
High milk production in summer.
What is colostrum
The first milk that a cow produces after calving. yellowish. Slaty liquid- very high serum protein and antibodies.
What is the effect of different milk compositions
Physico-chemical properties, functional properties, pricing of milk (prices based on the milk fat and protein solids )
What are the physical properties of milk
- appearance
- density
- osmotic pressure
- freezing point
- Ph and acidity
- Opacity- due to suspended particles of fat, proteins and certain minerals
colour-white to flight yellow (carotene content) - 1.028-1.033 g/cm3 20degrees C
- isotonic
- -0.512 to -0.59
- fresh milk=6.6-6.8, acidity- 0.13% lactic acid.
What does pH of the milk indicate
important indicator of microbial quality of raw milk- concentration of [H+] ions.
Lipids are soluble in non-polar organic solvents and insoluble in water- How does the milk fat exist?
potential SA
Milk is an oil in water emulsion, the fat exists as small globules or droplets dispersed in the milk serum. Each fat globule is enveloped by a (biological membrane) (the milk fat globule membrane- acts as an emulsifier).
What are the main classes of lipids found in milk?
Triacylglycerols (98.3%): glycerol backbone with 3 FA groups, phospholipids (0.8%)
which FA is unique to milk?
What are the most abundant FA
butyric acid- distinctive odour and taste.
C16 (palmitic), C18 (stearic) and C18:1 (oleic)
proportion of TAG varies with diet and lactation stage.
Why does butter not spread straight from the fridge?
It has a high melting temperature due to the high melting temperature of the FA found in largest proportions
Palmitic 25%- 62C
Oleic acid 30% -14C
stearic- 7% - 70C
What affects the crystallisation and positioning of the milk fat?
The distribution of FA’s over the position in TAG molecule.
Butyric and Caproic mostly 3rd
stearic mostly 1st.
What is milk fat
A mixture of different FA and glycerol= TAG
Milk fat globule membrane
- comes from?
- colour
- function
- plasma membrane of the mammary secretory cell
- reddish brown due to xanthine oxidase
- dispersion of fat in the aqueous phase of milk + fat protection from lipase.
what are the minor lipid components found in milk?
Sterols (95% is cholesterol), carotenoids (beta carotene is 95%), fat soluble vitamins (vit A, E, D and K)
What are the types of Milk proteins found in milk?
two major: caseins (~80%) and whey (~20%) proteins.
minor: membrane proteins
How does Casein exist in milk
exist as colloidal particles (casein micelles)
major and minor types of casein found in milk
major: alpha s1,alpha s2, beta and k-caseins (in approximate
ratio of 40:10:35:12); synthesized in secretory cells
minor: gamma and proteose peptone.
insoluble at pH 4.6 and 20C
How does casein exist in milk
Primarily as casein micelles stabilised by k-casein on surface
What stabilises the casein micelle?
Zeta surface potential, -20mV and steric stabilisation.
What is lactoferrin
A minor component of whey, equivalent to transferrin in blood. has antimicrobial properties. Binds iron. heat sensitive: denatures at about 65C.
What is Lactose
It is the carbohydrate in milk. It is a disaccharide made from glucose and galactose. It is the leat soluble of the common sugars.
What is a problem with lactose in the milk
It can cause defects in concentrated milk and frozen dairy products
What are the minerals found in milk
calcium, magnesium, potassium and sodium.
bicarbonate, chloride, citrate slats.
these are distributed between the soluble phase and colloidal phase. - minerals affect stability of milk and milk products + maintain osmotic P.
Importance of cooling milk?
Milk leaves the udder at a temperature of around 37C, it is continuously contaminated with microbes which start to multiply (ideal environment). The cooling the temperature of the milk the slower the rate of bacteria multiplication. Thus milk must be cooled to 4C immediately after milking. (must be transported under refrigerated conditions).
How is milk prepared for processing?
The Raw milk undergoes quality testing via: taste, smell , composition, antibiotics testing, freezing point, pH/ acidity.
How is milk standardised?
though fat and protein- adding or removing
How is milk fat separated out
In commercial dairy plants- uses centrifugal force.
How is milk protein separated out
Commercial dairy plants- membrane filtration (porous polymeric sheets).
definition of permeate
the filtrate- liquid passing though
definition of retentate
the concentrate, the retained liquid.
Different membrane processes in dairy industry
Reverse osmosis (concentration of solutions be removal of water),
nano filtration (concentration of organic components by removal of part of monovalent ions like sodium and chlorine)
Ultrafiltration: concentration of large and macro molecules (e.g. proteins)
micro filtration: removal of bacteria, separation of macro molecules
Dead-end filtration Vs cross flow filtration
dead end filtration= with gravity, on membrane. feed perpendicular to membrane
cross flow= feed parallel to membrane
What is permeate, why is it added?
All components of milk but protein in ultra filtration.
Added to ensure there is specific [] of proteins.
What is the purpose of homogenisation
Ensures that the fat stays evenly distributed though the milk. - Stabilising the fat against gravity separation- primarily causes disruption of fat globules into much smaller ones.
How does Homogenisation occur
Milk is forced though a small passage at high velocity. Breaks down the fat molecules into smaller pieces.
Advantages of Homogenisation (5)
- smaller fat globules leading to less cream- line formation
- whiter and more appetising colour
- reduced sensitivity to fat oxidation
- more full bodied flavour, and better mouthfeel
- better stability of cultured milk products
Disadvantages of homogenisation (2)
- somewhat increased sensitivity to light- sunlight and fluorescent tubes- can results in
‘sunlight flavour’ - the milk might be less suitable for production of semi-hard- hard cheese because the coagulum will be soft and difficult to dewater.
Purpose of heat processing of milk
- To destroy pathogenic and spoilage bacteria in milk.
- main bacteria when it first leaves udder is LA bacteria. during cold storage LAB do not grow but psychrotrophic bacteria do. These are main cause of spoilage (main type: Pseudomonas species)
- Reduce/ destroy enzyme activity present in milk
- Achieve desired shelf-life
Primary bacteria when milk first leave the udder?
lactic acid bacteria e.g. Lactococcus, lactobacillus
What bacteria grows in the cold?
psychrotrophic bacteria
What are the major cause of spoilage in milk
Psychrotrophs
main: Pseudomonas species.
What is spoilage in milk caused by
By enzymes produced by bacteria including protease, lipase, lactase (beta- galactosidase)
If not properly treated, what can occur?
An overgrowth of Psychrotrophs, lead to milkborne diseases such as turberculosis, brucellosis, and typhoid fever.
What heat treatment produces the longest shelf life? long long
UHT, months - can be kept at room temperature
How long can Pasteurized milk last
Days- must be chilled
How long can ESL milk last
Weeks- must be chilled
What are the five types of heat treatment of milk, in order of increasing severity?
- thermisation
- Pasteurisation
- High temperature Pasteurisation (ESL- extended shelf life)
- Ultra high temperature (UHT)
- In container sterilisation
What are some undesirable effects of heating milk
Loosing nutrients, change in colour, change/ loss in flavour
What is thermisation
60-65 degrees for 5-15 seconds
- reduces psychrotrophic bacterial count.
- Does not ensure destruction of pathogenic bacteria
- Not widely practised in Aus
What is Pasteurisation
The most effective control measure for eliminating pathogens that may be present in raw milk, including Lsteria monocytogens, Campylobacter spp., Salmonella spp., and pathogenic E.Coli.
- Most common heat process
- Shelf life can be 7,10, 14 or up to 16 days.
What are the pasteurisation regulations in Australia
Milk must be pasteurised by
(a) heating to a temp of no less then 72C and retaining for no less then 15seconds
(b) heating, using any other time and temperature combination of equivalent or greater lethal effect on any pathogenic micro-organisms in the milk.
What is a test that ensures that milk is properly pasteurised and safe for human consumption?
Alkaline phosphatase test. inactivated under the same conditions.
What are the two processes with equivalent sets of heating conditions
- Batch: Low temp, long time: 65degrees for 15 minutes
* continuous: high temperature, short time
What are the two processes with equivalent sets of heating conditions
- Batch: Low temp, long time: 65degrees for 15 minutes
* continuous: high temperature, short time. 72degrees C for 15 seconds.
What does batch method of pasteurisation involve
Uses a vat pasteurizer.
- consists of a jacketed vat
- surrounded by either circulating water, steam or heating coils or water or steam
- milk is heated and held throughout the holding period while being agitated
- milk may be cooled in the vat.
What does Continuous milk pasteurisation involve
High temp, short time.
uses plate heat exchanger.
* a stack of corrugated stainless steel plates clamped together in a frame.
*the heating medium: vacuum steam or hot water
* hot water on opposite sides of the plates heat milk to a temperature of at least 72degrees C.
* Flows through the holding tube where it is held for at least 16s.
* the warm milk passes through the cooling section where it is cooled to 4C
What is the shelf life of pasteurized milk? why is it not longer
12-16 days
It is not packaged aseptically- spoilage largely due to post-pasteurisation contamination of the milk by psychrotrophic bacteria. (major)
Does not kill some thermoduric organisms such as corynebacteria and the spore-forming bacillus species. (minor spoilage organisms)
What are the advantages of Pasteurisation?
- causes only minor changes to milk components. (little denaturation of whey proteins, very little destruction of vitamins, small loss of water soluble vit, small change in flavour).
- easy to test: using alkaline phosphatase test
- extends shelf life of raw milk
Definition of ESL
‘the ability to extend the shelf life of a chilled distributed product beyond the shelf life of a traditional pasteurised equivalents in a specific market.
limits recontamination faced by pasteurisation
What are the three types of processing technologies for ESL treatment.
Type of technology depends on the type of product and the desired outcome.
1) Pasteurization combined with bactofugation or double bactofugation
2) pasteurization combined with microfiltration
3) high heat treatment
ESL with high heat what are the heating conditions? packaging conditions storage conditions self life? designed to kill?
*heating conditions are between pasteurisation and UHT usually ~120-130 C for 2-5 s
*packaged under very clean but not aseptic conditions
*stored under refrigeration
* shelf life of several weeks- longer than pasteurised.
> not common in AUS
* designed to kill most thermoduric organisms including coryneforms and some sporeforming bacteria such as Bacillus species.
What is Tetra pak ESL?
micro filtration solutions
UHT
heating conditions
Heating conditions 130-150C for 3-5s.
can be stored at room temperature for ~6mthds
what are the main types of UHT processing?
- Direct heating
- milk that is mixed directly with steam, heating by transfer of latent heat from steam
- product held at elevated temp for a shorter period of time= less damage, less cooked flavour - Indirect heating
- milk is heated with heat exchanger: steam or hot water heats a stainless steel barrier which heats the milk.
No direct contact.
Draw the graph of direct and indirect continuous sterilization
- longer amount of time that could be damaged with indirect UHT