Cytoplasmic Organelles Flashcards

1
Q

Where does much of the energy generating metabolism occur in the cell?

A

Mitochondria, chloroplasts, and peroxisomes

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2
Q

What feature of mitochondria and chloroplasts have which allows them to encode their own genes?

A

They carry their own DNA

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3
Q

What feature of mitochondria and chloroplasts have which allows them to make some of their own proteins?

A

They carry their own ribosomes

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4
Q

How do mitochondria, chloroplasts, and peroxisomes divide?

A

Autonomously; binary fission

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5
Q

What does the endosymbiont hypothesis explain?

A

Explains the origins of eukaryotes from prokaryotes

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6
Q

What evidence backs up the endosymbiont hypothesis?

A

Mitochondria and chloroplasts:

  1. Have circular DNA
  2. Have inner membranes with prokaryote properties
  3. Have 70S ribosomes
  4. Have unique protein import mechanisms
  5. Divide by binary fission
  6. If chloroplasts are depleted from a cell, they cannot reform
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7
Q

What are mitochondria developed from?

A

Proteobacteria

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8
Q

What are chloroplasts developed from?

A

Cyanobacteria

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9
Q

Where are the proteins translated in the post-translational pathway?

A

On free ribosomes in the cytosol

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10
Q

What molecule helps the transport of proteins through organelle membranes?

A

Chaperonins

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11
Q

How many mitochondria are found in a cell?

A

20 - >1000 /cell

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12
Q

What is the name of the protein that forms the small pores found in the outer membrane of a mitochondrion?

A

Perforin

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13
Q

What size of molecules can diffuse through the pores in the mitochondria’s outer membrane?

A

Less than 10kDa

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14
Q

What do the integral proteins found in the mitochondria’s inner membrane do?

A

Principally required for transport and processes that lead to ATP production

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15
Q

What will you find in a mitochondrion’s matrix?

A
  1. Molecules that have been specifically transported across the membrane, or have been generated by the mitochondrion’s own DNA
  2. Mitochondrial DNA, RNA, ribosomes
  3. Enzymes for the Kreb’s cycle
  4. Small granules
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16
Q

How big are chloroplasts?

A

5 - 10µm

17
Q

What is the difference between the outer and the inner membrane of chloroplasts?

A

Outer - permeable to small molecules

Inner - impermeable to most molecules, inc. small ones

18
Q

How are thylakoids arranged?

A

In stacks called grana

19
Q

Where exactly does photosynthesis occur?

A

In the thylakoid membranes

20
Q

What occurs during photosynthesis?

A

The capture of photons
Electron transport and ATP production
Reduction of NADP+ to NADPH

21
Q

Where do light dependent and light independent (dark) reactions occur?

A

Light dependent - thylakoids

Light independent - stroma

22
Q

Where are photons absorbed?

A

By pigments (usually chlorophyll) in photocentres

23
Q

What happens during the Calvin cycle?

A

CO2 from the atmosphere is converted to 3-carbon sugar phosphates, which can then be converted to glucose

24
Q

How big are peroxisomes?

A

0.5µm

25
Q

How many peroxisomes are found in a cell? What kind of cell would contain more?

A

~500/cell, more in liver cells

26
Q

What are the 2 more well known functions of peroxisomes?

A

A compartment for oxidation reactions, and lipid synthesis

27
Q

What do the oxidation reactions in peroxisomes achieve?

A

They break down a variety of substrates, inc. uric acid, ethanol, and methanol

28
Q

What is the by-product of the oxidation reactions that occur in peroxisomes?

A

Hydrogen peroxide (hence the name peroxisome)

29
Q

What kind of lipids are peroxisomes involved in the synthesis of?

A

Cholesterol and bile acids (derived from cholesterol)

30
Q

What is the name of the phospholipid found in the cell membranes of Schwann cells? (Synthesised by peroxisomes)

A

Plasmalogen