Cytokine receptor

Question 1

What are the effector properties of cytokines

Answer 1

Pleiotropic(diff effects on diff types of target cells)
- Redundancy (multiple cytokines hv same effect)
- synergistics (cooperative effect)
- antagonistic (inhibition by one another)

Question 2

How many classes in cytokine recep

Answer 2

I (classical), II (TNF), III (IL-1)

Question 3

TNF full form

Answer 3

tumour necrosis factor

Question 4

How many types of class I cytokine recep

Answer 4

Type I and II

Question 5

How many types of type I classical

Answer 5

• Single chain recep fml
• Common y chain recess fml
• Gp130 recep fml
• common Bchain recep fml

Question 6

Exp of type I single chain recep

Answer 6

Erythropoietin

Question 7

Exp of type I common ychain recep

Answer 7

IL-2 fml + IL-4

Question 8

Exp of type I Gp130 recep

Answer 8

IL-6

Question 9

Exp of type 1 Bchain recep

Answer 9

IL-5

Question 10

Exp of Type II classical recep

Answer 10

IFNy

Question 11

Diff btw type I and II classical

Answer 11

Type I has conserved WSXWS motif on extracellular domain

Question 12

How classical cytokine recep activated by erythropoietin

Answer 12

dimerisation, JAK-STAT pathway activated

Question 13

What happen when erythropoietin recep ACTIVATED

Answer 13

JAK-2 phosphorylates Tyr on intracellular domain of EpoR
- Activated Tyr as docking sites of STAT5 via its SH2 domain
- STAT5 phosphorylated by JAK2, form dimers
- STAT5 activates gen transcription

Question 14

Tm for Rheumatoid arthritis

Answer 14

Oral Tofacitinib high potency in inhibiting JAK1 & 3, prevent STAT translocation into nucleus

Question 15

Tofacitinib side effect

Answer 15

• Increased risk of shingles infection
• Anaemia, when JAK2 activity affected

Question 16

JAK-1 specific inhibitor

Answer 16

Abrocitinib

Question 17

JAK-STAT regulation

Answer 17

• SHP-1 removes P from JAK
• PIAS binds to activated STAT prevent DNA binding
• SOCS produced by STAT activated gene transcription, binds to Tyr-P on JAK/receptor
• SUMO binds to STAT affect its stability

Question 18

Exp of Class II (TNFR)

Answer 18

TNFR1, RANK

Question 19

RANK full form

Answer 19

Receptor activator of NF-kappaB

Question 20

Ligand of TNF1 and RANK

Answer 20

TNFa, RANKL

Question 21

What are the two pathways TNFR1 can activate

Answer 21

Inflammatory and death-inducing

Question 22

Pathways of inflammatory TNFR1

Answer 22

MAPK activation, NF-kappaB activation

Question 23

Pathway of death-inducing pathway of TNFR1

Answer 23

Pro-caspase 8/10 activation