Cytogenetic techniques Flashcards
1
Q
Southern blotting
A
Specific Sequence in DNA
2
Q
Western blotting
A
Detection of specific protein after electrophoresis
3
Q
Northern blotting
A
Specific RNA after electrophoresis
4
Q
PCR
A
Amplify DNA (need to know DNA sequence)
3 steps - double stranded genomic DNA is denatured by heat into single stranded DNA, DNA polymerase used to extend the primers, cycling is set to produce necessary number
5
Q
FISH
A
Cytogenetic technique to detect and localize specific DNA sequence on chromosomes
6
Q
DNA cloning
A
DNA fragment can be isolated and inserted into the genome of self-replicating plasmids
