CYTOGENETIC TECHNIQUES Flashcards
the chromosomes are arranged based on which of the following?
a. length
b. location of centromere
c. banding pattern
d. all of these
D
using the g-banding techniques, which of the following is true of the g-band?
a. pale staining
b. euchromatic
c. rich in adenine and thymine
d. bright bands
C
which of the following types of specimen is routinely used for adult and children in classical cytogenetics?
a. bone marrow
b. heparinized peripheral blood
c. skin bodies
d. kidney
B
which of the following reagents is used to halt cell division in cell culture in classical cytogentics?
a. colcemid
b. phytoemgglutinin
c. carnoy’s reagent
d. 0.075M KCl
A
B is for mitosis
C is for fixation
D is for swelling of chromosomes
which of the following banding techniques is the reverse of g-banding?
a. Q
b. T
c. R
d. C
C
which of the following is not true of FISH?
a. can detect presence or absence of specific DNA sequence in chromosomes
b. uses fluorescent dyes as label
c. uses dna probes on a chromosome spread
d. hybridization demonstrated by autoradiography
D
which of the following molecular techniques can distinguish the 24 chromosomes?
a. whole chromosome painting
b. spectral karyotyping
c. multicolor FISH
d. all telomere FISH
B
which of the following is not an advantage of FISH over classical cytogenetics?
a. more sensitive in detecting subtle changes in chromosome feature
b. does not require living cells
c. can be quantified automatically
d. labor intensive
D (LESS labor intensive)
which of the following hybridization techniques can detect copy number variation of a DNA sequence?
a. FISH
b. CGH
c. aCGH
d. multicolor FISH
B
which of the following molecular techniques can detect hundreds to thousands of features in DNA sequence?
a. microarray hybridization
b. FISH
c. CGH
d. spectral karyotyping
A
