Current trends on Forensic STR analysis Flashcards
more loci
applied bio systems global filler
24 loci
improved sensitivity
more alleles = more info
pick up more background DNA
issue with improved sensitivity
can lead to contamination issues
can make interpretation more difficult
improved inhibitor tolerance
Promega PowerPlex Fusion system
How has the PCR cycle time changed?
faster
2.5 hours to 50 mins
Rapid DNA
IntegenX RapidHIT system
profiles now being loaded to NDNAD
how long does IntegenX RapidHIT system take?
90 mins from sample to profile
what 4 things does IntegenX RapidHIT system involve?
extraction, amplification, electrophoresis and analysis
higher information content
Reluctance to move away from STRs as all current systems and databases are based on them
Next generation sequencing allows other markers to be analysed alongside STRs
mtDNA, Y=STRs, SNPs
STR sequence as well as length – mixture deconvolution
Large amount of information – bioinformatics
Need to work out how to incorporate and report all the new info
how many countries have implemented national programs?
49
by 2025, the number of reference DNA profiles in databases is predicted to rise to what
100-150 million
what country announced in 2015 compulsory DNA testing for all residents?
Kuwait
cross-border sharing of DNA information
Terrorism, migration, cross-border crime
Prüm convention in Europe
Allows searching of DNA databases between member countries
European standard STR loci
direct to consumer DNA testing
The rise of recreational DNA testing
Family history and ethnicity testing
