current trends in forensic sTR analysis Flashcards
1
Q
more loci
A
- Applied biosystems globalfiler (24 loci)
2
Q
improved sensitivity
A
- More alleles= more information
- But improved sensitivity can lead to contamination issues
- Pick up more background dna, can make interpretation more difficult
3
Q
improved inhibitor tolerance
A
- Promega Powerplex fusion system
4
Q
faster results
A
- PCR cycling time is coming down
- 2.5 hours 50 minutes
- Rapid DNA systems
- Urgent as standard
5
Q
rapid DNA
A
- IntegenX RapidHIT system
- ~ 90 mins from sample to profile
- Extraction, amplification, electrophoresis and analysis in one
- Mobile system, taking analysis out of the lab
- Profiles now being loaded to the NDNAD
6
Q
Higher information content
A
- Reluctance to move away from DTRs as all current systems and databases are based on them
- Next generation sequencing allows other markers to be analysed alongside STRs
- mtDNA, Y-STRs, SNPs
- STR sequence as well as length- mixture deconvolution
- Large amount of information- bioinformatics
- Need to work out how to incorporate and report all the new information
7
Q
DNA databases
A
- By 2025 the number of reference DNA profiles in databases is predicted to ris to ~100-150 million
- Several large countries are close to implementing nation DNA databases: Brazil, South Africa, Vietnam, Thailand, India
- In July 2015, Kuwait announced compulsory DNA testing for all residents
- Anyone who doesn’t comply could face a large fine and 1 year in prison
8
Q
cross border sharing of DNA information
A
- Terrorism, migration, cross border crime
- Prum convention in Europe
- Allows searching of DNA databases between member countries
- European standard STR loci
9
Q
direct to consumer DNA testing
A
- The rise of “recreational” DNA testing
- Family history and ethnicity testing
- Who owns your DNA?
- What is it being used for?
- Golden state killer case
