culturing microorganisms Flashcards
1
Q
how are microorganisms cultured on a small scale
A
in a petri dish with agar gel
2
Q
why should you work near a bunsen burner?
A
carries bacteria or microbes upwards by causing an air current
3
Q
state 5 aseptic techniques
A
- regularly disinfect surfaces
- wash hands
- pass neck of broth bottle through bunsen flame
- sterilise equipment in flame of bunsen
- use disposable pre sterilised plastic instruments
4
Q
why is the neck of the broth bottle passed through flame?
A
- heats the air
- air expands and causes outflow
- prevents microbes / bacteria falling in
5
Q
why does tape no cover entire plate?
A
- allow some oxygen to enter
- for aerobic respiration
- whilst allowing lid to be secure
