Culturing and harvesting techniques

Question 1

The following enzymes can be used to dissociate Chorionic villus Samples
(CVS) EXCEPT FOR: (2A2)
a) RNAse
b) Collagenase
c) Trypsin
d) Protease

Answer 1

a) RNAse

Question 2

Which of the following is not a necessary (must be added) ingredient
which is added to modified medium? (2A4)
a) L-Glutamine
b) Vitamins
c) Antibiotics
d) Buffer

Answer 2

c) Antibiotics

Question 3

All of the following have buffering capability EXCEPT: (2A4)
a) Sodium bicarbonate
b) L-Glutamine
c) Hepes
d) FCS

Answer 3

b) L-Glutamine

Question 4

What type of media is necessary for the culture of Fragile X cases? (2A5)
a) Media with extra FCS
b) Folic acid deficient media
c) Complete media
d) Lysine rich media

Answer 4

b) Folic acid deficient media

Question 5

The most important reason why Biological Safety Cabinets are used
instead of Horizontal Laminar Flow Hoods in the handling of human
tissues is: (2B1)
a) To provide a clean working area
b) To prevent contamination of the specimen.
c) To protect the technologist who is processing the specimen.
d) To prevent cross-contamination between two different cell lines

Answer 5

c) To protect the technologist who is processing the specimen.

Question 6

Which of the following can be used for serial culture from amniotic fluid?
(2C2)
a) Erythrocyte
b) Lymphocyte
c) Epithelial cells
d) Fibroblast

Answer 6

d) Fibroblast

Question 7

Which of the following chemicals is used for cryopreservation? (2D2)
a) Methanol
b) Ethyl Alcohol
c) Dimethyl sulfoxide (DMSO)
d) Sodium hypochlorite

Answer 7

c) Dimethyl sulfoxide (DMSO)

Question 7

Adding the drug Colcemid to cell cultures can cause all of the following
phenomenon to occur EXCEPT: (2G4)
a) Chromosome condensation
b) Increase in the number of metaphases
c) Cell swelling
d) Depolymerization of the spindle apparatus

Answer 7

c) Cell swelling

Question 8

During slidemaking all the following factors are important EXCEPT FOR:
(2H1)
a) Drying rate (Evaporation)
b) Quality of the glass slides
c) Slides are first dipped in water
d) Slides are first dipped in acetic acid

Answer 8

d) Slides are first dipped in acetic acid

Question 9

A good banding pattern will be expected when we observe chromosomes
before banding under the phase scope with the following appearance: (21)
a) Dark
b) Pale
c) Shiny
d) Separated chromatids

Answer 9

a) Dark

Question 10

Which of the following factors are not important for chromosome
spreading? (2J)
a) Hypotonic treatment time
b) Dropping the cell from an increased height
c) Cell suspension concentration
d) Mitotic index

Answer 10

d) Mitotic index

Question 11

PHA is a common additive to blood cultures. What category of additive is it? (2A4)
a) a mitogen
b) an anticoagulant
c) growth serum
d) arresting solution

Answer 11

a) a mitogen

Question 12

On Monday morning the technologist noticed widespread cell death in incubator A. He checked the environment and conditions ofthe cultures. What condition would likely be the cause of the cell
death? (2F)
a) 5% C02 atmosphere
b) 40 °C incubator temperature
c) a pH of 7.3
d) all conditions would cause cell death

Answer 12

b) 40 °C incubator temperature

Question 13

Which media component is unstable? (2A4)
a) Serum
b) L-glutamine
c) Antibiotics
d) amino acids

Answer 13

b) L-glutamine

Question 14

)What is the purpose of establishing a PHA-stimulated blood culture for a leukemia patient? (2C)
a) To check for circulatingimmature cells in the peripheralblood.
b) To see if an abnormality is constitutional rather than acquired.
c) To look for early rearrangements whichmay have predisposedthis individualto developcancer.
d) To study leukemic cells ofT-cell origin

Answer 14

b) To see if an abnormality is constitutional rather than acquired.

Question 15

Whatis the purpose of establishing an unstimulated bloodculture for a leukemia patient? (2A4)
a) To check for circulating immature cells in the peripheral blood.
b) To see if an abnormalityis constitutional rather than acquired.
c) To look for earlyrearrangements whichmayhavepredisposed thisindividual to develop cancer.
d) all ofthe above

Answer 15

a) To check for circulating immature cells in the peripheral blood.

Question 16

After what period of time do PHA peripheral blood lymphocytes reach their first division?(2C)
a) 12 hours
b) 24 hours
c) 36 hours
d) 48 hours

Answer 16

d) 48 hours

Question 17

Which of the following is information thatisimportant to consider when freezing cells? (2D)
a) Age ofthe culture
b) Concentration of cells
c) Cell type
d) Media used

Answer 17

b) Concentration of cells

Question 18

24 BM and 72 BM LPS cultures would be set up on a new CLL with a wbc of 5x 106. (2A6)
a) True
b) False

Answer 18

a) True

Question 19

PHA will affect which ofthe following types of cells? (2A4)
a) B-cells
b) Platelets
c) T-cells
d) Phagocytes

Answer 19

c) T-cells

Question 20

Which ofthe following may cause cell death: (2F)
a) A five percent C02 atmosphere
b) A 24 hour delay in culturing
c) A pH of 7.2
d) Incubation at a temperature of 40°C

Answer 20

d) Incubation at a temperature of 40°C

Question 21

For Optimal cell growth, which ofthe following ph will provide that? (2C2)
a) 6.8 - 7.0
b) 7.0-7.2
c) 7.2 - 7.4
d) 7.4-7.6

Answer 21

c) 7.2 - 7.4

Question 22

Which ofthe following tissues grows most slowly in culture? (2C2)
a) Fetal tissue
b) Skin
c) Gonad
d) Amniotic fluid cells

Answer 22

b) Skin

Question 23

All ofthe following ARE characteristics of the horizontal laminar flow hood EXCEPT? (2B1)
a) Protects the culture from contamination
b) Should not be used with transformed cell lines.
c) Protects the worker from contamination
d) Sterilizes the air entering the hood

Answer 23

c) Protects the worker from contamination

Question 24

Which ofthe following media supplements is optional for long term cultures? (2A4)
a) Insulin
b) Antibiotics and antifungicides
c) Growth factors
d) Fatal calfserum

Answer 24

b) Antibiotics and antifungicides

Question 25

Which ofthe following best describes a media that has a low serum level? (2A4)
a) Defined
b) Maintenance
c) Growth
d) Complete

Answer 25

b) Maintenance

Question 26

The statements below refer to culturing methods. Which statementis FALSE: (2A)
a) The preferred mode for cytogeneticanalysisof solidtumors is long term culture.
b) The success rate of cytogenetic analysis of solidtumors is lessthanthat of leukemias currently.
c) Culture of epithelial tumors is more difficult than other types of tumors.
d) A Biosafety Cabinet is required for tissue culture.

Answer 26

a) The preferred mode for cytogeneticanalysisof solidtumors is long term culture.

Question 27

Regarding the following statements, which is FALSE? (2C)
a) Epithelial cells cannot bemorphologically distinguished from mesenchymal cells inculture when
viewed under an inverted phase contrast microscope.
b) Contamination with normal fibroblasts can give anerroneous result.
c) Different media are usedin the culture of different tumor types.
d) All ofthe above

Answer 27

a) Epithelial cells cannot bemorphologically distinguished from mesenchymal cells inculture when

Question 28

Harvest ofa 14 day old amniotic fluid culture results inmany cells, but few mitoses. The most likely
cause ofthe low mitotic rate is: (2F)
a) Senility ofthe culture.
b) Chromosome abnormality
c) Maternal age
d) Contactinhibition due to high cell confluence

Answer 28

d) Contact inhibition due to high cell confluence

Question 29

The culture method used to obtain chromosome preparations from amniotic fluid is considered: (2A1)
a) Short term culture
b) Intermediate term culture
c) Long term
d) None ofthe above

Answer 29

a) Short term culture

Question 30

This type of culture has a decreased turn around time and allows easier distinction between mosaicism
and pseudomosaicism. (2A1)
a) Primary amniotic fluid cell culture
b) In situ-amniotic fluid culture
c) Flask-amniotic fluid culture
d) Short term amniotic fluid culture

Answer 30

b) In situ-amniotic fluid culture