Chromosome banding technique

Question 1

Study the differences between banding techniques

Answer 1

Question 2

G banding study card

Answer 2

Question 3

The best method to process slides for G-banding on the same day that
they are dropped is: (3A1)
a) Warm the last fix to 37 C during harvest.
b) Prolong the trypsin treatment time.
c) Incubate the slide in 60 C for 1-3 hrs before banding.
d) Increase the Giemsa concentration

Answer 3

c) Incubate the slide in 60 C for 1-3 hrs before banding.

Question 4

You are analyzing a clinical case and discover a small extra marker with
satellites. What staining technique would be most useful in determining
the chromosomal origin of this marker? (3B4)
a) QFQ
b) GTG
c) DAPI/DA
d) NOR

Answer 4

c) DAPI/DA

Question 5

When using a fluorescein labeled probe during the FISH procedure, the
appropriate counterstain used should be: (3B5)
a) 5-Bromodeoxyuridine
b) Ethidium bromide
c) Propidium iodide
d) DAPI

Answer 5

c) Propidium iodide

Question 6

When doing sequential staining which of the following is used to remove
oil from slides? (3D)
a) Ether
b) Xylene
c) Acetic acid
d) Methanol

Answer 6

b) Xylene

Question 7

What is the best banding technique to distinguish between 47,XY,+18 and
47,XYY? (3A2)
a) QFQ
b) GTG
c) DAPI/DA
d) RHG

Answer 7

a) QFQ

Question 8

When the identification of paired chromosomes is the primary goal, which
of the following banding techniques should be used? (3A1)
a) Q-banding
b) G-banding
c) Giemsa staining
d) C-banding

Answer 8

b) G-banding

Question 9

When the addition of nonhomologous material to the q-arm of
chromosome 16 is suspected after G-banding, which of the following
banding techniques should be used? (3B2)
a) C-banding
b) Q-banding
c) R-banding
d) NOR staining

Answer 9

a) C-banding

Question 10

Which chromosome is most different between C and G banding? (3B2)
a) 1
b) 9
c) 16
d) Y

Answer 10

b) 9

Question 11

What is not a criteria for G-band identification (3A1)
a) band level
b) location of bands
c) location of centromere
d) location of telomere

Answer 11

d) location of telomere

Question 12

What does quinacrine and acridine orange dyes stain? (3A2)
a) histones
b) nonhistones
c) GC rich DNA
d) AT rich DNA

Answer 12

d) AT rich DNA

Question 13

Exam question

Q banding and G banding difference, if a band is dark in G banding what color is it in Q banding?

Answer 13

Dark
G and Q and C banding stain the same, R banding is the reverse banding and is opposite