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Culture Media Flashcards

1
Q

Prevent the access of micro organisms during the preparation and testing

A

Aseptic Technique

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2
Q

With application of safranin, grwm negative bacteria appear color

A

Pink or Red

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3
Q

The substance introduced or added by Carl Weigert in Staining

A

Safranin

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4
Q

In gram staining, gran negative bacteria shows what color before application of safranin

A

Colorless at first

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5
Q

In gram staining, gram positive bacteria shows what color

A

Dark Purple

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6
Q

He developed Gram Staining

A

Christian Gram

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7
Q

The first and usually the only method of staining employed for the diagnostic identification of bacteria un clinical specimens

A

Gram’s Staining

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8
Q

A staining also known as Ziehl Neelsen. It is a modification of Ehrlich original method of differential staining for bacilli

A

Acid Fast Staining

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9
Q

A stain that provides color contrast but give same color to all bacteria and cells

A

Simple staining

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10
Q

Staining that provides different color to different bacteria

A

Differential staining

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11
Q

A staining method that uses acidic dye to stain the background of a specimen, making the specimen itself visible

A

Negative Staining

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12
Q

A staining method where actual cells are themselves colored and appear in a clear background

A

Positive staining

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13
Q

A type of staining where both positively and negatively charged imparts different colors to different components

A

Neutral Staining

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14
Q

A type of staining that involves positively charged______radicals combines with negatively charged particles in cytoplasm and gives color

A

Basic Staining

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15
Q

A type of staining that involves negatively charged acid radicals that imparts color in eosin, acid fuschine, malachite green, nigrosin, indian ink

A

Acidic Staining

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16
Q

A type of culturing that involves flooding the surface of a plate with a liquid suspension of bacteria. It is used fir bacteriophage typing and antibiotic sensitivity testing

A

Lawn Culture

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17
Q

A type bacteria culturing where agar is melted and cooled to 45°C then 1ml inoculum is added. It is used for estimated CFU

A

Pour plate culture

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18
Q

A type of culturing that involves a puncturing a medium with a wire

A

Stab Culture

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19
Q

A type of culturing where organisms are inoculated on a slant medium or agar slope

A

Stroke Culture

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20
Q

It is commonly streaking method used for isolating colonies

A

Quadrant method

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21
Q

A type of culturing that is used for isolation of bacteria in pure culture from clinical specimen

A

Streak culture

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22
Q

Media used to grow anaerobic organisms

A

Anaerobic media

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23
Q

It is used as temporary storage of specimens being transported which typically contains only buffer and salt

A

Transport Media

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24
Q

A media containing any fermentable substance

A

Sugar Media

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25
McLeod's media is an example of
Indicator media
26
A type of indicator media where Salmonella typhi forms black colonies
Wilson Blair Medium
27
Wilson Blair media is an example of
Indicator media
28
Contains an indicator which changes its color when a bacterium grows in them
Indicator media
29
A differential media used to isolate and enumerate urinary pathogens
CLED - Cysteine Lactose Electrolyte Deficient Agar
30
A media that differentiate between lactose and sucrose fermenting bacteria
Eosin Methylene Blue
31
In McConkey agar, non lactose fermenters are shown in what color
White/Pale colonies
32
In MacConkey agar, lactose fermenters are shown in what color
Red colors
33
A media designed to grow gram negative bacteria useful in cultivation of enterobacteriaceae
McConkey Agar
34
Mannitol salt agar is an example of
Differential media
35
Eosin Methylene Blue is an example of
Differential media
36
When a media contains substance which helps distinguish differing characteristics of bacteria, it is called
Differential media
37
TCBS is an example of
Selective Media
38
It is used to grow Vibrio Cholerae where bile salts inhibit the growth of unwanted bacteria
TCBS Thiosulfate Citrate Bile Salts
39
It is used to cultivate Mycobacterium spp and commonly used in the lab
Lowenstein Jensen Media
40
It is used to isolate pathogenic Neisseria spp
Thayer Martin Agar
41
Lowenstein Jensen medium is an example of
Selective media
42
Thayer Martin Agar is an example of
Selective media
43
Salmonella Shigella agar is an example of
Selective Media
44
McConkey Agar is an example of
Selective media and differential media
45
Selective media suppresses the growth of other species by incorporating
dyes, bile salts, adjust pH
46
It is a media in which certain substance are present which inhibit all other bacteria except the desired bacteria
Selective media
47
Media originally formulated for isolation of Neisseria spp and Moraxella spp
Mueller Hinton agar
48
It is useful in antibiotics testing and starch hydrolysis testing
Mueller Hinton Agar
49
Mueller Hinton agar is an example of
Enriched media
50
Brain heart infusion agar is an example of
Enriched media
51
Chocolate agar is an example of
Enriched media
52
it is obtained form RBC breakdown and are different medium for gram positive cocci
Chocolate agar
53
Bloog agar is an example of
Enriched media
54
Composed of sterile nutrient agar + 5-10% sheep blood and are red in color
Blood agar
55
Blood agar media and chocolate agar are examples of
Enriched media
56
Used for cultivation of all fastidious microorganisms such as Haemophiles influenza and streptococcus spp
Enriched media
57
When basal medium is added with some nutrients such as blood serum or egg, it is called
Enriched media
58
pH of supportive media
5.5-7.7
59
Nutrient broth and tryptic soy agar are examples of
Supportive media
60
It contains nutrients that support and sustain growth of many organisms
Supportive media
61
Growth factor of culture media that functions as coenzyme
Vitamins
62
Growth factor requirements of culture media responsible for nucleic acid synthesis
Purine and Pyrimidine
63
Growth factor of culture media responsible for the protein synthesis
Amino acid
64
Suitable pH requirements of culture media
7.2-7.4
65
Required carbonates of culture media includes
Potassium, Magnesium, Cobalt
66
Mineral salt requirements of culture media
Sulphates, Phosphates, Chlorides
67
Nutrient requirements of culture media
Source of energy, carbon, and nitrogen
68
Also known as synthetic or defined medium
Biphasic Blood Culture Media
69
Media recommend if brucellosis is suspected
Biphasic Blood Culture Media
70
Has both liquid and solid phase also known as Castaneda medium
Biphasic Blood Culture Media
71
Melts at 90°C above, remains liquid at 42°C and solidifies at 40°C Below
Agar
72
It is used in medium and has no nutritive value and not affected by the growth of bacteria
Agar
73
Golden yellow granular powder
Agar
74
Ingredients of media that is prepared from fresh lean meat
Meat extract
75
Ingredients of media that is prepared from cells of Baker's yeast or saccharomyces. Used as vitamin source
Yeast
76
Ingredients of media that acts as buffer, hygroscopic in nature, and is also a nitrogen source
Peptones
77
ingredients of media that acts as vehicle
Water( Distilled)
78
Type of culture media with 0.5% agar and has jelly consistency
Semisolid
79
Type of culture media that contains 2% agar. Used for isolation of bacteria as a pure colonies
Solid
80
Type of culture media that allows bacteria to grow uniformly with turbidity. No agar
Liquid
81
He used cooked potato pieces as a solid medium to grow bacteria
Robert Koch
82
He used simple broth made up of urine and meat extract
Louis Pasteur
83
A special medium containing all the required nutrients used to grow different kinds of microorganisms in microbiology laboratories
Culture media
84
A special medium containing all the required nutrients used to grow different kinds of microorganisms in microbial laboratories
Culture media
85
A method of sterilization used for enclosed area such as inoculation room and virus labs
Radiation
86
A method of sterilization used for heat sensitive liquids like antibiotics solutions and sera
Filtration
87
A method of sterilization uses steam under high pressure such as autoclaving of culture media
Moist heat
88
A method of sterilization uses hot air oven glassware and flaming of inoculation loop and needles
Dry heat
89
It is a process by which the growth of bacteria is inhibited but they are not killed
Antisepsis
90
It is a process by which an article, surface or medium is made free from all pathogenic microorganisms that are capable of giving rise to infections
Disinfection
91
It is a process by which surface or medium is made free from all microorganisms either in vegetative or spore state
Sterilization
92
It prevents the access of microorganisms during the preparation and testing
Aseptic technique
93
Refers to a procedure that is performed under sterile conditions
Aseptic technique

WEEK 1 (4 decks)

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