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CULTURE MEDIA Flashcards

ppt/trans based

1
Q

also known as Agar-agar

A

Agar

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2
Q

“In vitro”

A

outside of the body

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3
Q

agar comes from

A

Red algae

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4
Q

cultivating microorganisms in vitro provides the appropriate biochemical and biophysical environment for microbial growth

A

Cultivation

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4
Q

agar consistency

A

gelatin-like consistency

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5
Q

material/substance to be inoculated in the agar

A

Inoculum

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6
Q

types of culture

A

pure
mixed
stock

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7
Q

virign/single species of
organisms)

A

pure

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8
Q

more than 2 organisms

A

mixed

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9
Q

for storage/maintained in order to keep microorganisms viable/alive

A

stock

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10
Q

classification of agar according to:

A

PHYSICAL STATE
CONSISTENCY
COMPOSITION
HOW THE MEDIUM IS DISPENSED AND USE

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11
Q

types of colony

A

Mucoid colony
Smooth Colony
Rough Colony

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12
Q

sticky/wet

A

Mucoid colony

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12
Q

Dull, dry

A

Rough Colony

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12
Q

Shiny, glistening surface

A

Smooth Colony

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12
Q

contains 0.5-1.5% of agar

A

SEMISOLID

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12
Q

what bacteria has condom shaped colonies

A

Mycobacterium

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12
Q

classification of culture media according to physical state/consistency

A

LIQUID
SEMISOLID
SOLID

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12
Q

without any trace of agar or solidifying substance

A

LIQUID

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13
Q

contains 2-3% of agar

A

SOLID

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14
Q

ACCORDING TO COMPOSITION

A

SYNTHETIC
NON-SYNTHETIC
TISSUE CULTURE MEDIUM

15
Q

Medium in which all components are known

16
Q

Medium which some of the substances are
unknown

A

NON-SYNTHETIC

17
Q

Obligate intracellular bacteria

A

TISSUE CULTURE MEDIUM

18
ACCORDING TO DISPENSING OR DISTRIBUTION
PLATED TUBED
19
Tube media are prepared as either LIQUID, SLANT, BUTT AND SLANT, or BUTT
TUBED
20
manner of inoculation in triple sugar iron
Slant and butt
21
manner of inoculation in urea
slant streak
22
manner of inoculation in lysine iron sugar
Stab Streak Stab
23
manner of inoculation in simmon's citrate
slant
24
manner of inoculation in christensen's urea agar
slant
25
what color does cua produce when it is positive
pink
26
manner of inoculation in sulfide indole motility
stab
27
production of sulfide (color)
black
28
indole (color)
red rings in top
29
motility
turbid agar
30
● Supports most non-fastidious bacteria ● for primary isolation of microorganism ● Designed to grow a broad spectrum of microbes
basal media
31
has blood, serum, egg, yolk, vitamins, AA ● Supports growth of fastidious organisms ● Solid culture bacteria
ENRICHED
32
blood type that is used in enriched media
Type O
33
blood in heat causes lysis making its color brown
Chocolate agar plates
34
Promotes growth of certain organisms Liquid culture bacteria
ENRICHMENT
35
contains inhibitory agents that prevents / suppress growth of unwanted microbes permits preliminary identification of genus or spp.
SELECTIVE
36
Make it easy to distinguish colonies of different microbes
DIFFERENTIAL MEDIA
37
complete hemolysis (yellow)
beta hemolysis
37
THREE HEMOLYTIC PATTERNS
beta hemolysis alpha hemolysis gamma hemolysis
38
incomplete/partial hemolysis
alpha hemolysis
39
no hemolytic pattern
gamma hemolysis
40
used for yeasts and molds for counting
Potato Dextrose Agar
40
SPECIMEN TRANSPORT
CARY-BLAIR AMIES TRANSGROWTH STUART
40
Used for specimens that are sensitive for drying
SPECIMEN TRANSPORT
41
used for AST [Antimicrobial Susceptibility Testing] to determine if bacteria is drug-resistant or drug-susceptible
Mueller-Hinton agar (MHA)

BACTE LAB (4 decks)

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