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BACTE LAB > CULTURE MEDIA > Flashcards

CULTURE MEDIA Flashcards

ppt/trans based

1
Q

also known as Agar-agar

A

Agar

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2
Q

“In vitro”

A

outside of the body

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3
Q

agar comes from

A

Red algae

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4
Q

cultivating microorganisms in vitro provides the appropriate biochemical and biophysical environment for microbial growth

A

Cultivation

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4
Q

agar consistency

A

gelatin-like consistency

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5
Q

material/substance to be inoculated in the agar

A

Inoculum

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6
Q

types of culture

A

pure
mixed
stock

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7
Q

virign/single species of
organisms)

A

pure

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8
Q

more than 2 organisms

A

mixed

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9
Q

for storage/maintained in order to keep microorganisms viable/alive

A

stock

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10
Q

classification of agar according to:

A

PHYSICAL STATE
CONSISTENCY
COMPOSITION
HOW THE MEDIUM IS DISPENSED AND USE

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11
Q

types of colony

A

Mucoid colony
Smooth Colony
Rough Colony

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12
Q

sticky/wet

A

Mucoid colony

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12
Q

Dull, dry

A

Rough Colony

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12
Q

Shiny, glistening surface

A

Smooth Colony

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12
Q

contains 0.5-1.5% of agar

A

SEMISOLID

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12
Q

what bacteria has condom shaped colonies

A

Mycobacterium

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12
Q

classification of culture media according to physical state/consistency

A

LIQUID
SEMISOLID
SOLID

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12
Q

without any trace of agar or solidifying substance

A

LIQUID

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13
Q

contains 2-3% of agar

A

SOLID

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14
Q

ACCORDING TO COMPOSITION

A

SYNTHETIC
NON-SYNTHETIC
TISSUE CULTURE MEDIUM

15
Q

Medium in which all components are known

16
Q

Medium which some of the substances are
unknown

A

NON-SYNTHETIC

17
Q

Obligate intracellular bacteria

A

TISSUE CULTURE MEDIUM

18
Q

ACCORDING TO DISPENSING OR DISTRIBUTION

A

PLATED
TUBED

19
Q

Tube media are prepared as either LIQUID, SLANT, BUTT AND SLANT, or BUTT

20
Q

manner of inoculation in triple sugar iron

A

Slant and butt

21
Q

manner of inoculation in urea

A

slant
streak

22
Q

manner of inoculation in lysine iron sugar

A

Stab
Streak
Stab

23
Q

manner of inoculation in simmon’s citrate

24
Q

manner of inoculation in christensen’s urea agar

25
Q

what color does cua produce when it is positive

26
Q

manner of inoculation in sulfide indole motility

27
Q

production of sulfide (color)

28
Q

indole (color)

A

red rings in top

29
Q

motility

A

turbid agar

30
Q

● Supports most non-fastidious bacteria
● for primary isolation of microorganism
● Designed to grow a broad spectrum of microbes

A

basal media

31
Q

has blood, serum, egg, yolk, vitamins, AA
● Supports growth of fastidious organisms
● Solid culture bacteria

32
Q

blood type that is used in enriched media

33
Q

blood in heat causes lysis making its color brown

A

Chocolate agar plates

34
Q

Promotes growth of certain organisms
Liquid culture bacteria

A

ENRICHMENT

35
Q

contains inhibitory agents that prevents / suppress
growth of unwanted microbes
permits preliminary identification of genus or spp.

36
Q

Make it easy to distinguish colonies of different microbes

A

DIFFERENTIAL MEDIA

37
Q

complete hemolysis (yellow)

A

beta hemolysis

37
Q

THREE HEMOLYTIC PATTERNS

A

beta hemolysis
alpha hemolysis
gamma hemolysis

38
Q

incomplete/partial hemolysis

A

alpha hemolysis

39
Q

no hemolytic pattern

A

gamma hemolysis

40
Q

used for yeasts and molds for counting

A

Potato Dextrose Agar

40
Q

SPECIMEN TRANSPORT

A

CARY-BLAIR
AMIES
TRANSGROWTH
STUART

40
Q

Used for specimens that are sensitive for drying

A

SPECIMEN TRANSPORT

41
Q

used for AST [Antimicrobial Susceptibility Testing] to determine if bacteria is drug-resistant or drug-susceptible

A

Mueller-Hinton agar (MHA)

BACTE LAB (4 decks)

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