ACID-FAST BACILLI STAINING Flashcards
trans based
common bacteria in acid-fast bacilli staining
Mycobacteria
cell wall have increased lipid
content
Mycolic acid
T/F: mycobacteria resists acid
T
primary stain of afbs
CARBOLFUCHSIN
secondary stain of afbs
METHYLENE BLUE
MALACHITE GREEN
mordant of Ziehl Neelsen
Method
HEAT
mordant of Kinyoun
Method
TERGITOL
principle of acid fast bacilli staining
The primary stains binds to mycolic acid in the cell walls of acid fast bacteria and is retained after decolorizing with acid alcohol
what color does afb retain
deep-pink or red colored
what color does non afb retain
blue or green colored.
hot method
Ziehl-Neelsen Method
“Cold” Method
Kinyoun Method
Not routinely used method
Auramine-Rhodamine Method
is needed because fluorochrome is needed as the reagent in order for us to perform this method.
Fluorescent microscope
who initially modified the stain
FRANZ ZIEHL
who discovered tubercle bacilli
Sir Robert Koch
He used Carbolfuchsin and heat –
however this was not enough
franz ziehl
who modified the stain with the addition of acid alcohol
Friedrich Neelsen
decolorizer of afbs
Acid Alcohol
color of afb+
RED/PINK
color of afb-
BLUE
PURPLE
GREEN
size of template
2cmx3cm
how do you know if your sample is a sputum
<10 epithelial cells, >25 polymorphonuclears
how do you know if your sample is a saliva
> 10 epithelial cells, <25
polymorphonuclears
