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BACTE LAB > ACID-FAST BACILLI STAINING > Flashcards

ACID-FAST BACILLI STAINING Flashcards

trans based

1
Q

common bacteria in acid-fast bacilli staining

A

Mycobacteria

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2
Q

cell wall have increased lipid
content

A

Mycolic acid

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3
Q

T/F: mycobacteria resists acid

A

T

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4
Q

primary stain of afbs

A

CARBOLFUCHSIN

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5
Q

secondary stain of afbs

A

METHYLENE BLUE
MALACHITE GREEN

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6
Q

mordant of Ziehl Neelsen
Method

A

HEAT

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7
Q

mordant of Kinyoun
Method

A

TERGITOL

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8
Q

principle of acid fast bacilli staining

A

The primary stains binds to mycolic acid in the cell walls of acid fast bacteria and is retained after decolorizing with acid alcohol

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9
Q

what color does afb retain

A

deep-pink or red colored

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10
Q

what color does non afb retain

A

blue or green colored.

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11
Q

hot method

A

Ziehl-Neelsen Method

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12
Q

“Cold” Method

A

Kinyoun Method

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13
Q

Not routinely used method

A

Auramine-Rhodamine Method

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14
Q

is needed because fluorochrome is needed as the reagent in order for us to perform this method.

A

Fluorescent microscope

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15
Q

who initially modified the stain

A

FRANZ ZIEHL

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16
Q

who discovered tubercle bacilli

A

Sir Robert Koch

17
Q

He used Carbolfuchsin and heat –
however this was not enough

A

franz ziehl

18
Q

who modified the stain with the addition of acid alcohol

A

Friedrich Neelsen

19
Q

decolorizer of afbs

A

Acid Alcohol

20
Q

color of afb+

A

RED/PINK

21
Q

color of afb-

A

BLUE
PURPLE
GREEN

22
Q

size of template

A

2cmx3cm

23
Q

how do you know if your sample is a sputum

A

<10 epithelial cells, >25 polymorphonuclears

24
Q

how do you know if your sample is a saliva

A

> 10 epithelial cells, <25
polymorphonuclears

25
Q

what is MDR-TB

A

multi-drug resistant tuberculosis

26
Q

how many drugs does mdr-tb resistant

A

2

27
Q

what is XDR-TB

A

extreme drug-resistant tuberculosis

28
Q

Skin test, injected in the arms

A

MANTOUX TUBERCULIN TEST

29
Q

reagent of mantoux tuberculin test

A

Purified Protein Derivative

30
Q

Stain used for Colorblind individuals

A

BAUMGARTEN’S STAIN

31
Q

2-4% NaOH
■ Sputolysin

A

Digestant

32
Q

when should you collect a sputum samplen and why

A

First morning specimen
2-3 specimens needed
because it is concentrated

32
Q

2-4% NaOH
■ Zephiran

A

Decontaminant

BACTE LAB (4 decks)

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