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CULTURE & CULTURE MEDIA Flashcards

1
Q

Pathogens in Gastrointestinal tract

A

SALMONELLA & SHIGELLA

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2
Q

lactose fermenters, and non-lactose
fermenters

A

MacConkey

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3
Q

hemolytic patterns
(streptococci)

A

BAP (Blood Agar Plate) -

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4
Q

Selects the growth of a particular organism at the same time Inhibiting the growth of other organisms.

A

SELECTIVE CULTURE MEDIA

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5
Q

Streptococci /Bacitracin

A

Gentamycin blood agar

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6
Q

Haemophilus

A

Chocolate agar

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7
Q

Aeromonas

A

Blood agar plate w/ ampicillin

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8
Q

Gram (+) cocci

A

Phenyl ethyl alcohol

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9
Q

gram (+) bacteria

A

Columbia CNA with blood

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10
Q

Salmonella spp and Shigella
spp

A

Gram negative (GN) broth

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11
Q

Neisseria gonorrhea

A

Thayer Martin Agar (TV)

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12
Q

a nutritionally rich formula containing three peptone sources and 5% defibrinated (fibrin has been removed) sheep blood

A

COLUMBIA CNA WITH BLOOD/Columbia agar base

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13
Q

refers to the antibiotics colistin (C) and nalidixic acid (NA) that are added to the medium to suppress the growth of most gram-negative organisms while allowing gram-positive
bacteria to grow

A

CNA

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14
Q

-an antibiotic that targets the bacterial structure of cell membrane and cell wall
- disrupt the cell membrane of gram negative organisms thus it will not grow

A

Colistin

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15
Q

blocks DNA replication of susceptible organism

A

Nalidixic acid

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16
Q

used for the cultivation of gastrointestinal pathogens from stool specimens and rectal swabs (for Salmonella and Shigella)

A

GRAM-NEGATIVE BROTH

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17
Q

contains sodium citrate and sodium deoxycholate (a bile salt) that inhibit gram-positive organisms and the early multiplication
of gram-negative, non enteric pathogens

A

GRAM-NEGATIVE BROTH

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18
Q

Both selective and Enrichment Broth

A

GRAM-NEGATIVE BROTH

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19
Q

Primary carbon source of gram-negative broth

A

MANNITOL

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20
Q

provides nutrients for common gram-positive
cocci such as enterococci, streptococci, and staphylococci

A

PHENYLETHYL ALCOHOL AGAR (PEA)

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21
Q

should not be used in the interpretation of hemolytic reactions

A

PHENYLETHYL ALCOHOL AGAR (PEA)

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22
Q

can ONLY be used for hemolytic patterns

A

BAP

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23
Q

inhibitory to gram negative

A

CRYSTAL VIOLET
BASIC FUCHSIN
BILE SALT

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24
Q

inhibitory to gram positive

A

POTASSIUM TELLURITE
SODIUM AZIDE

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25
Q

inhibitory to swarming phenomenon

A

ALCOHOL
CHLORAL HYDRATE

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26
Q

SELECTIVE CULTURE MEDIUM FOR NEISSERIA

A

Thayer Martin Medium
Martin Lewis Medium
New York City Medium

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27
Q

SELECTIVE CULTURE MEDIUM FOR STAPHYLOCOCCUS

A

Mannitol Salt Agar
Phenyl Ethyl Alcohol Agar
Vogel and Johnson Agar
Chapman Stone Agar

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28
Q

SELECTIVE CULTURE MEDIUM FOR GROUP A
STREPTOCOCCUS

A

Crystal Violet Blood Agar
Polymyxin B, Neomycin acid media
(PNF)

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29
Q

SELECTIVE CULTURE MEDIUM FOR GROUP B
STREPTOCOCCUS

A

Lim, Carrot Broth
Todd and Hewitt Broth with Antibiotics
Granada Agar

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30
Q

SELECTIVE CULTURE MEDIUM FOR VIBRIO

A

Thiosulfate Citrate Bile Salt Sucrose
Monsur Tellurite Taurocholate Gelatin
Agar

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31
Q

isolate bacteria with specific growth requirements

A

SPECIAL MEDIA

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32
Q

medium for selective media

A

Lowenstein-Jensen (L-J) medium

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33
Q

TCBS meaning

A

Thiosulfate Citrate Bile Salt Sucrose

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34
Q

specialized medium to isolate Mycobacterium tuberculosis

A

Lowenstein-Jensen (L-J) medium

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35
Q

Selective and differential medium used for isolation and identification of Vibrio cholera

A

Thiosulfate Citrate Bile Salt Sucrose (TCBS)

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36
Q

act as sulfur source

A

Thiosulfate

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37
Q

buffering agent of TCBS

A

Citrate

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38
Q

Inhibit the growth of non-vibrio species

A

Bile Salts

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39
Q

Fermentable sugar that allows for the differentiation of Vibrio species based on their ability to ferment sugar

A

Sucrose

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40
Q

Used when there is an anticipated delay in bringing the specimen into the laboratory

A

TRANSPORT MEDIUM

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41
Q

how many minutes can a transport medium hold a specimen?

A

30 minutes

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42
Q

Transport medium of Streptococcus pyogenes

A

PIKE’S MEDIA

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43
Q

Transport medium of Neisseria

A

JEMBEC

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44
Q

Transport medium of Vibrio cholera

A

Alkaline Salt Transport Medium

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45
Q

Transport medium of Salmonella typhi

A

Glycerol Saline Transport Media

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46
Q

Transport medium of Bordetella

A

Mishulow’s Medium

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47
Q

-Used to demonstrate the antibiotic resistance or sensitivity
- an organism to different antibiotics

A

CULTURE MEDIUM FOR SENSITIVE/SUSCEPTIBILITY TESTING

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48
Q

for fastidious organisms

A

Mueller Hinton Agar (MHA)

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49
Q

pH of MHA

50
Q

Depth of MHA

A

at least 4 mm

51
Q

Culture medium for sensitivity of Mycobacterium

A

Middlebrook ZH 10, 7H 11

52
Q

Culture medium for sensitivity of anaerobic bacteria

A

Wilkin-Chalgren Agar

53
Q

Used to demonstrate biochemical activities of bacteria that is useful in their identification

A

BIOCHEMICAL MEDIUM

54
Q

special culture medium used to facilitate anaerobic cultivation

A

THIOGLYCOLATE AND CYSTEINE

55
Q

used to the culture medium to remove (drive off) oxygen to facilitate anaerobic cultivation

56
Q

Remove residual oxygen to facilitate anaerobic cultivation

A

Anaerobic chamber system with a vacuum pump and nitrogen gas

57
Q

convert oxygen to water to facilitate anaerobic cultivation

A

Gaspak jar containing hydrogen and palladium catalysts

58
Q

Used for small volume to facilitate anaerobic cultivation

A

plastic bag or pouch containing calcium
carbonate and catalyst

59
Q

Color of Methylene blue in the absence of oxygen

60
Q

remove oxygen from the chamber by combining with hydrogen to form water

A

Palladium pellets

61
Q

diagnostic purposes when infectious disease are involved

A

CULTURE and CULTURE MEDIA

62
Q

Understand patterns of microbial metabolism in the laboratory

A

CULTURE and CULTURE MEDIA

63
Q

Develop methods for interrupting their spread and controlling their growth

A

CULTURE and CULTURE MEDIA

64
Q

Process of cultivating and growing of bacteria in a controlled environment

65
Q

involved providing necessary nutrients, temperature, and other condition in order to support their growth

66
Q

commonly used in industries like biotechnology and pharmaceuticals

67
Q

where antibiotics came from

68
Q

provide necessary nutrients like CHO, CHON, salts, and vitamins

A

CULTURE MEDIA

69
Q

2 MAIN TYPES OF CULTURE MEDIA

A

SOLID MEDIA & LIQUID MEDIA

70
Q

can be added; not one of the main type

A

SEMI-SOLID

71
Q

material containing the necessary nutritional and environment requirements for bacterial growth

A

CULTURE MEDIUM

72
Q

can be liquid, semi-solid or solid preparation utilized to observe growth pattern of microorganism as well as for transport and storage

A

CULTURE MEDIUM

73
Q

3 TYPES OF CULTURE

A

PURE CULTURE
MIXED CULTURE
STOCK CULTURE

74
Q

One genus

A

PURE CULTURE

75
Q

pour the broth of the organism in the agar

A

pour plate

75
Q

inoculating in agar plate until you can see a single colony

A

streak plate

76
Q

you’ll get in the mixed culture and if you put it in the selective media, it only allows to grow what it wants to grow

A

selective media

77
Q

organisms are injected in the animals

A

animal inoculation

78
Q

more than 2 genus and species

A

MIXED CULTURE

79
Q

for academic and industrial purposes

A

STOCK CULTURE

80
Q

3 CLASSIFICATION OF CULTURE MEDIA
According to PHYSICAL STATE or CONSISTENCY

A

LIQUID FORM
SEMI-SOLID
SOLID

81
Q

Broth, infusion, milk, Brain heart infusion

A

LIQUID FORM

82
Q

-Allows growth of aerobes, anaerobes and facultative anaerobes
-Nutrients needed are going to dissolve in water
-Bacterial growth indicates by change in appearance

A

LIQUID FORM

83
Q

if something grows, it will become turbid
(positive)

A

NUTRIENT BROTH

84
Q

a sulfated polymer made of D-galactose, 3,6 anhydro-L-galactose, and D glucuronic acid and usually derived from red algae

85
Q

-Clot like consistency
- used for motility medium, transport medium

A

SEMI-SOLID FORM

86
Q

doesn’t need oxygen but if you put them in semi-solid form, they usually grow in the bottom

A

STRICT ANAEROBES

87
Q

they will grow or multiply near the surface

88
Q

% of agar in Semi-Solid Form

A

0.5% - 1.0% agar

89
Q

% of agar in Solid Form

90
Q

Provides a firm surface on which organisms can form colonies

A

SOLID FORM

91
Q

Melting point of agar in solid form

A

80 - 90 degrees celsius

92
Q

Solid Form solidifies at what temp?

A

40 - 50 degrees celsius

93
Q

2 CLASSIFICATION OF CULTURE MEDIA According to the manner of DISPENSING or DISTRIBUTION

A

PLATED
TUBED

94
Q

2 CLASSIFICATION OF CULTURE MEDIA According to COMPOSITION

A

Synthetic or defined medium
Non-synthetic or complex medium
Tissue culture medium

95
Q

All substances are known to the uses
- used for research purposes

A

SYNTHETIC/DEFINED MEDIUM

96
Q

Composed of some unknown substances
- Very useful for the isolation of bacteria

A

NON-SYNTHETIC or COMPLEX MEDIUM

97
Q

To isolate obligate intracellular bacteria

A

TISSUE CULTURE MEDIUM

98
Q

organisms that do not require any special conditions and substances for their growth

A

Non-fastidious organisms

99
Q

Contains nutrients required to support the growth of wide range/variety of organism

A

ENRICHED CULTURE MEDIUM

100
Q

For most fastidious bacteria (requires special requirement)

A

BLOOD AGAR

101
Q

Enriched medium for Haemophilus and Neisseria

A

CHOCOLATE AGAR

102
Q

2 Enriched culture medium

A

BLOOD AGAR
CHOCOLATE AGAR

103
Q

most frequently used for primary and selective and differential agars so it contains crystal violet to inhibit the growth of the gram positive bacteria and fungi

A

CHOCOLATE AGAR

104
Q

also known as Hemin

105
Q

NAD or NADH

106
Q

Enumerate the Preferred blood for the preparation of BAP

A

1st: Sheep
2nd: Horse
3rd: Rabbit

107
Q

contains non-specific inhibitors

A

Human Blood

108
Q

HUMAN BLOOD CONTAINS

A

CITRATE
DEXTROSE

109
Q

inhibit growth of (-hemolytic streptococcus)

110
Q

alter types of hemolysis

111
Q

Increases the number of pathogens that are outnumbered by non-pathogens

A

ENRICHMENT CULTURE MEDIUM

112
Q

Extends the lag phase of non-pathogens while decreasing the lag phase of pathogens

A

ENRICHMENT CULTURE MEDIUM

113
Q

allows or support the growth wide range of organism with additional nutrient

A

ENRICHED MEDIA

114
Q

with additional nutrient but encourages specific group of organism

A

ENRICHMENT MEDIA

115
Q

enrichment broth or semisolid media

A

THIOGLYCOLATE BROTH

116
Q

contains many nutrient factors, including casein, yeast and beef extracts, and vitamins

A

THIOGLYCOLATE BROTH

117
Q

Distinguishes group of organisms based on cultural characteristics

A

DIFFERENTIAL CULTURE MEDIUM

118
Q

allows visualization of metabolic differences between group of species of bacteria

A

DIFFERENTIAL CULTURE MEDIUM

119
Q

contains bile salts and dyes (bromothymol blue and acid fuchsin) to selectively slow the growth of most nonpathogenic gram-negative bacilli found in the gastrointestinal tract

A

HEKTOEN ENTERIC AGAR (HEA)

120
Q

indicator for the detection of H2 S-producing organisms like Salmonella spp., can be visualized as colonies exhibiting a black precipitate

A

Ferric ammonium citrate

BACTERIOLOGY PRELIMS (3 decks)

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