CULTURAL CHARACTERISTICS Flashcards
major features of bacteria
APPEARANCE
very important in bacterial identification
factors of bacterial colony
4 COLONY SIZE (MEASURED IN MILLIMETERS)
Pinpoint
Small
Medium
Large
refers to the coloration observed in bacterial or fungal colonies grown in agar plates
COLONY PIGMENTATION
bacteria that give a green metallic sheen on
EMB agar
Escherichia coli
Forms (Colony shape)
Punctiform
Circular
Filamentous
Irregular
Rhizoid
Curled
Elevation (Colony elevation)
Crateriform
Raised
Flat
Umbonate
Convex
Margin of Colony
Entire (smooth)
Irregular
Filamentous
Glistening and yellow
Circular
glistening, and white
Irregular
veined, and rough, yellow
Rhizoid
rough, and white
Filamentous
COLONY SURFACE APPEARANCE
Glistening
Smooth
Dull
Wrinkle
Rough
observed in Blood Agar
Hemolytic Pattern
colony sinks in the agar
Pitting of the agar surface
grape like odor
Pseudomonas aeruginosa
Color
White
Yellow
Black
Buff
Cream
Density
Opaque
Translucent
Transparent
CONSISTENCY
Butyrous
Viscid
Membranous
Brittle
required to measure the size of the agar colonies
SIZE
Punctiform size agar plate colonies
< 1 mm
Small size in agar plate colonies
1-2 mm
Medium size in agar plate colonies
3-4 mm
Large size in agar plate colonies
> 5 mm
spread across the entire agar plate
Pseudomonas and Proteus
evenly circular or with irregularities
MARGIN OR EDGE
Colonies may either be flat or raised
ELEVATIONS
Not all bacterial species have this distinctive feature
CHROMOGENESIS
May be diffusible to non-diffusible
CHROMOGENESIS
Some bacteria retained the pigment within the cell while other bacteria colored the medium
CHROMOGENESIS
May be opaque, translucent or opalescent
OPTICAL FEATURES
put on tube not on the petri dish
GROWTH ON AGAR SLANT
Amount (2)
Scanty
Moderate or Abundant
MARGIN OR EDGE (4)
- evenly circular or with irregularities
- Rounded projections
- Irregular notches
- Threadlike or root-like projections
CONSISTENCY OF MASS GROWTH (3)
- butyrous or butter-like,
- viscous or stringy
- dry and brittle
Talks about the turbidity of the nutrient broth
DISTRIBUTION IN BROTH