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Culture And Culture Media Flashcards

1
Q

A culture of a staphylococcus aureus only, is considered to be what type of culture?

A

Pure Culture

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2
Q

growth of microorganism

A

Culture

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3
Q

It is composed of more than one species

A

Mixed culture

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4
Q

Composed of several species contained in a separate culture medium

A

Stock culture

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5
Q

TRUE OR FALSE. stock culture’s shelf life should be at least 12 months and an organism should be subcultured twice after thawing to return it to a healthy state.

A

True 😛

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6
Q

What type of culture is used for academic and industrial purposes?

A

Stock culture

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7
Q

It allows the growth of aerobes, anaerobes and facultative anaerobes. It is also prone from contamination

A

Liquid medium

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8
Q

Liquid medium is composed of ___% agar

A

0% agar

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9
Q

What is the indicator for the growth of bacteria in a liquid medium?

A

Turbidity of the medium

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10
Q

A semi-solid medium contains __-__% agar

A

0.5-1% agar

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11
Q

SIM medium is used to identify microorganism’s ______. What does SIM stands for?

A

Motility; SULFIDE INDOLE MOTILITY

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12
Q

It is the most commonly used medium. It contains 2%-3% agar

A

Solid medium

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13
Q

Enumerate 3 liquid medium.

A

✔️Brain heart infusion
✔️trypticase soy broth
✔️thioglycollate

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14
Q

T OR F. Triple sugar iron is a solid media placed in a tube?

A

True

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15
Q

At this range of temperature, agar will melt.

A

80-90*C

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16
Q

What temperature does an agar solidify?

A

40-50C

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17
Q

ture or false. It takes 65-70*C to cool an agar.

A

False. Bc the cooling temperature of an agar is 55-60 C

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18
Q

It is a medium in which all of the components are known to the user and it is used for research purposes

A

Synthetic/Defined medium

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19
Q

The composition is unknown, though it is very useful for the isolation of bacteria.

A

Non-synthetic/complex medium

20
Q

Used for obligate intracellular bacteria

A

Tissue culture medium

21
Q

Enumerate the different types of tube medium.

A

✔️Butt
✔️Butt and slant
✔️Slant
✔️Liquid

22
Q

It is routinely used without added supplement. It also supports he growth of most nonfastidious bacteria.

A

Simple/general purpose/supportive media

23
Q

A simple/general purpose/supportive media is composed of a ____& ____ extracts.

A

Meat and soybean extracts

24
Q

it propagates the growth of certain group of bacteria and it is a liquid type.
It is also used as supplement to agar plates

A

Enrichment media

25
Q

Media with added supplement necessary for the growth of fastidious organisms.

A

Enriched media/nonselective media

26
Q

Allows visualization of metabolic differences

A

Differential media

27
Q

Media incorporated with chemicals, dyes or antibiotics to inhibit the growth of other organisms while promoting the growth of the desired organism.

A

Selective media

28
Q

Selective media for streptococci

A

Gentamicin blood agar

29
Q

Selective media for hemophilus

A

Bacitracin chocolate agar

30
Q

Selective media for aeromonas

A

Blood agar plate with ampicillin

31
Q

Selective media for g+ cocci

A

Phenyl ehtyl alcohol

32
Q

Selective media for g+ bacteria

A

Columbia agar with cna

33
Q

Selective media for salmonella ans shiegella spp.

A

Gram-negative broth

34
Q

Selective media for neisseria gonorrheae

A

Thayer Martin Agar

35
Q

Isolate bacteria with specific growth requirements

A

Special media

36
Q

Most commonly performed inoculation technique

37
Q

It is used ti differentiate the hemolytic patterns. Also used for fastidious organisms.

A

Blood Agar

38
Q

It is composed of a mixture of nutrients utilized to observe growth patterns if microorganisms

A

Culture medium

39
Q

Inhibitory substances for g+ bacteria

A

Crystal/gentian violet
Basic/carbol fuchsin
Bile salt

40
Q

Inhibitory substances of G- bacteria

A

Sodium azide and potassium tellurite

41
Q

Inhibitory substances of swarming bacteria

A

Alcohol and chloral hydrate

42
Q

What media do you use for the isolation of mycobacteria?

A

Lowenstein Jensen medium

43
Q

True or false. The specimen on swab is applied by stabbing the swab.

A

False, because the swab should be applied by rolling the swab onto a small area at the end of the plate, and then streak.

44
Q

What is the manner of reporting of agar-plate colonies?

A 
Size
Margin or edge
Elevation
Chromogenesis
Optical features
45
Q

Manner of reporting griwth on agar slant.

A

Amount
Margin or edge
Consistency or mass growth
Chromogenesis

46
Q

Manner of reporting of growth in nutrient broth

A

Amount
distribution in broth
Odor

47
Q

Manner of reporting of the growth in gelatin stabs

A

Growth along the line of inoculation

Liquefaction of gelatin

Microbiology (2 decks)

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