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IMSE - Complement Pathway > CRP > Flashcards

CRP Flashcards

1
Q

2 major parts of Acute Phase Reactants?

A

C-Reactive Proteins and Serum Amyloid A

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2
Q

CRP is an indicative of?

A

Inflammation

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3
Q

It is a type of cell that synthesizes our Acute Phase Reactants?

A

Hepatocytes

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4
Q

It is a polypeptide that activates our Acute Phase Reactants?

A

Cytokines

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5
Q

Vacutainer tube used in CRP determination?

A

Green top, heparinized blood, heparin

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6
Q

Principle of the CRP Determination?

A

Reverse Passive Agglutination based on the latex-agglutination method

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7
Q

In this reaction, the antibody is attached to a?

A

Carrier particles

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8
Q

True of False:

These carrier particles are used in Reverse
Passive Agglutination and should have the ability to absorb the FC region of the antibody. The FC region is the fragment crystallizable art of the antibody.

A

True

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9
Q

Positive indication of CRP determination?

A

Agglutination or visible clumping

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10
Q

6 types of Agglutination?

A

Direct Agglutination
Passive Agglutination
Reverse-Passive Agglutination
Agglutination Inhibition
Co-agglutination
AHG mediated agglutination reaction

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11
Q

Test commonly used in direct agglutination?

A

Blood typing

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12
Q

This uses carrier particles such as erythrocytes, latex particles, and charcoal particles.

A

Passive Agglutination

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13
Q

Serologic tests that under passive agglutination which is for systemic lupus erythematosus?

A

Antinuclear Antibody

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14
Q

2 serologic test under passive agglutination?

A

Antistreptolysin O (ASO)
Antinuclear Antibody

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15
Q

This uses bacteria as carrier particles?

A

Co-agglutination

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16
Q

There’s a competition between the soluble antigen and soluble antibody that happens on the body binding site.

A

Agglutination Inhibition

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17
Q

This agglutination reaction is dealing with the sensitization of RBC and usually encountered in BLOOD BANKING?

A

AHG mediated agglutination reaction

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18
Q

Serologic test such as BLOOD-TYPING is done with this kind of agglutination?

A

Direct Agglutination

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19
Q

Reagents of the CRP Determination Test?

A

CRP Latex Reagent
CRP Positive Control
CRP Negative Control
Glycine-Saline Buffer

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20
Q

Concentration dilution of Glycine-Saline Buffer?

A

1:20 with Distilled Water

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21
Q

CRP Latex Reagent contains?

A

Polystyrene Latex particles coated with anti-human CRP in stabilized buffer with <0.1% sodium azide as preservative

22
Q

CRP Positive control contains?

A

Human serum that contains >6mg/L CRP and <0.1% sodium azide as preservative.

23
Q

CRP Negative control contains?

A

Human serum diluted and stabilized with buffer and contain <0.1% sodium azide as preservative.

24
Q

In one test card, we just need to obtain serum only for about?

A

40 uL of serum

25
Q

QC visualizes?

A

Positive control

26
Q

Interpretation of result?

A

Positive - Agglutination
Negative - smooth milky suspension

27
Q

Negative result should be rule out with?

A

Serial dilution

28
Q

Negative result may be caused by?

A

Antigen excess or Prozone effect

29
Q

Prozone effect can cause?

A

False-Negative result

30
Q

CRP is a trace constituent of serum originally thought to be an antibody to the?

A

C-polysaccharide of pneumococci

31
Q

CRP was discovered by?

A

Tillet and Francis in 1930

32
Q

RP is known to have a more generalized role in?

A

Innate Immunity which is second line of defense.

33
Q

CRP is a relatively stable serum protein with a half-life of about?

A

18 hours

34
Q

True or False:

If there’s inflammation, our C-reactive protein is the first possible to increase.

A

True

35
Q

It increases rapidly within _______ following infection, surgery, or other trauma to the body.

A

4-6 hours

36
Q

CRP can exceed its peak value within the _______ mark. It could be ______, thousand folds

A

48 hours and 100 folds

37
Q

Elevated levels are found in conditions such as? 7 conditions.

A

-Bacterial infections
-Rheumatic fever
-Viral infections
-Malignant diseases
-Tuberculosis
-After a heart attack.
-The median CRP value for an individual increases with age, reflecting an increase in subclinical inflammatory conditions.

38
Q

True or False:

CRP is a nonsurgical means of following the course of malignancy and organ transplantation because a rise in the level may mean a return of the malignancy or, in the case of transplantation, the beginning of organ rejection.

A

True

39
Q

Assays for CRP are? 3 types.

A

Sensitive
Reproducible
Inexpensive

40
Q

CRP is easily destroyed by?

A

heating serum to 56°C for 30 minutes.

41
Q

CRP concentration of less than 1 mg/L is associated with a?

A

low risk for cardiovascular disease

42
Q

1 to 3 mg/L is associated with an?

A

Average risk

43
Q

greater than 3 mg/L is associated with a?

A

High risk.

44
Q

Normal levels in adults range from approximately?

A

0.47 to 1.34 mg/L

45
Q

A mean for people with no coronary artery disease is?

A

0.87 mg/L

46
Q

Correct disposing of controls?

A

CRP Positive control > CRP Negative control > Human serum

47
Q

The product is in Vitro or Vivo Diagnostic?

A

In Vitro Diagnostic

48
Q

Product should be approved by?

A

U.S. FDA

49
Q

They should be non-reactive for the presence of?

A

HbsAg (Hepatitis B Virus)
HIV (Human Immunodeficiency Virus)

50
Q

Prior to use, allow reagents and controls to warm up to room temperature and let it aside for about?

A

2-3 minutes

IMSE - Complement Pathway (2 decks)

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