CRP

Question 1

2 major parts of Acute Phase Reactants?

Answer 1

C-Reactive Proteins and Serum Amyloid A

Question 2

CRP is an indicative of?

Answer 2

Inflammation

Question 3

It is a type of cell that synthesizes our Acute Phase Reactants?

Answer 3

Hepatocytes

Question 4

It is a polypeptide that activates our Acute Phase Reactants?

Answer 4

Cytokines

Question 5

Vacutainer tube used in CRP determination?

Answer 5

Green top, heparinized blood, heparin

Question 6

Principle of the CRP Determination?

Answer 6

Reverse Passive Agglutination based on the latex-agglutination method

Question 7

In this reaction, the antibody is attached to a?

Answer 7

Carrier particles

Question 8

True of False:

These carrier particles are used in Reverse
Passive Agglutination and should have the ability to absorb the FC region of the antibody. The FC region is the fragment crystallizable art of the antibody.

Answer 8

True

Question 9

Positive indication of CRP determination?

Answer 9

Agglutination or visible clumping

Question 10

6 types of Agglutination?

Answer 10

Direct Agglutination
Passive Agglutination
Reverse-Passive Agglutination
Agglutination Inhibition
Co-agglutination
AHG mediated agglutination reaction

Question 11

Test commonly used in direct agglutination?

Answer 11

Blood typing

Question 12

This uses carrier particles such as erythrocytes, latex particles, and charcoal particles.

Answer 12

Passive Agglutination

Question 13

Serologic tests that under passive agglutination which is for systemic lupus erythematosus?

Answer 13

Antinuclear Antibody

Question 14

2 serologic test under passive agglutination?

Answer 14

Antistreptolysin O (ASO)
Antinuclear Antibody

Question 15

This uses bacteria as carrier particles?

Answer 15

Co-agglutination

Question 16

There’s a competition between the soluble antigen and soluble antibody that happens on the body binding site.

Answer 16

Agglutination Inhibition

Question 17

This agglutination reaction is dealing with the sensitization of RBC and usually encountered in BLOOD BANKING?

Answer 17

AHG mediated agglutination reaction

Question 18

Serologic test such as BLOOD-TYPING is done with this kind of agglutination?

Answer 18

Direct Agglutination

Question 19

Reagents of the CRP Determination Test?

Answer 19

CRP Latex Reagent
CRP Positive Control
CRP Negative Control
Glycine-Saline Buffer

Question 20

Concentration dilution of Glycine-Saline Buffer?

Answer 20

1:20 with Distilled Water

Question 21

CRP Latex Reagent contains?

Answer 21

Polystyrene Latex particles coated with anti-human CRP in stabilized buffer with <0.1% sodium azide as preservative

Question 22

CRP Positive control contains?

Answer 22

Human serum that contains >6mg/L CRP and <0.1% sodium azide as preservative.

Question 23

CRP Negative control contains?

Answer 23

Human serum diluted and stabilized with buffer and contain <0.1% sodium azide as preservative.

Question 24

In one test card, we just need to obtain serum only for about?

Answer 24

40 uL of serum

Question 25

QC visualizes?

Answer 25

Positive control

Question 26

Interpretation of result?

Answer 26

Positive - Agglutination Negative - smooth milky suspension

Question 27

Negative result should be rule out with?

Answer 27

Serial dilution

Question 28

Negative result may be caused by?

Answer 28

Antigen excess or Prozone effect

Question 29

Prozone effect can cause?

Answer 29

False-Negative result

Question 30

CRP is a trace constituent of serum originally thought to be an antibody to the?

Answer 30

C-polysaccharide of pneumococci

Question 31

CRP was discovered by?

Answer 31

Tillet and Francis in 1930

Question 32

RP is known to have a more generalized role in?

Answer 32

Innate Immunity which is second line of defense.

Question 33

CRP is a relatively stable serum protein with a half-life of about?

Answer 33

18 hours

Question 34

True or False: If there’s inflammation, our C-reactive protein is the first possible to increase.

Answer 34

True

Question 35

It increases rapidly within _______ following infection, surgery, or other trauma to the body.

Answer 35

4-6 hours

Question 36

CRP can exceed its peak value within the _______ mark. It could be ______, thousand folds

Answer 36

48 hours and 100 folds

Question 37

Elevated levels are found in conditions such as? 7 conditions.

Answer 37

-Bacterial infections -Rheumatic fever -Viral infections -Malignant diseases -Tuberculosis -After a heart attack. -The median CRP value for an individual increases with age, reflecting an increase in subclinical inflammatory conditions.

Question 38

True or False: CRP is a nonsurgical means of following the course of malignancy and organ transplantation because a rise in the level may mean a return of the malignancy or, in the case of transplantation, the beginning of organ rejection.

Answer 38

True

Question 39

Assays for CRP are? 3 types.

Answer 39

Sensitive Reproducible Inexpensive

Question 40

CRP is easily destroyed by?

Answer 40

heating serum to 56°C for 30 minutes.

Question 41

CRP concentration of less than 1 mg/L is associated with a?

Answer 41

low risk for cardiovascular disease

Question 42

1 to 3 mg/L is associated with an?

Answer 42

Average risk

Question 43

greater than 3 mg/L is associated with a?

Answer 43

High risk.

Question 44

Normal levels in adults range from approximately?

Answer 44

0.47 to 1.34 mg/L

Question 45

A mean for people with no coronary artery disease is?

Answer 45

0.87 mg/L

Question 46

Correct disposing of controls?

Answer 46

CRP Positive control > CRP Negative control > Human serum

Question 47

The product is in Vitro or Vivo Diagnostic?

Answer 47

In Vitro Diagnostic

Question 48

Product should be approved by?

Answer 48

U.S. FDA

Question 49

They should be non-reactive for the presence of?

Answer 49

HbsAg (Hepatitis B Virus) HIV (Human Immunodeficiency Virus)

Question 50

Prior to use, allow reagents and controls to warm up to room temperature and let it aside for about?

Answer 50

2-3 minutes