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BIS205: Genes, genomes and chromosomes > CRISPR-Cas and targeting bacterial pathogens > Flashcards

CRISPR-Cas and targeting bacterial pathogens Flashcards

1
Q

What is the CRISPR-Cas system in bacteria?

Clustered Regularly Interspaced Short Palindromic Repeats

A

Bacterial defense system against bcteriophages
Small interfering RNA system

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2
Q

What is the structure of a CRISPR loci?

A

-cas (CRISPR-associated) gene upstream of repeat spacer array
-short (~30bp) direct repeats separated by spacers (20-72bp of phage DNA captured from previous attacks)

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3
Q

Transformation efficiency =

A

number of colonies/mass of DNA

∴ transformation efficiency unit is cfu/μg

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BIS205: Genes, genomes and chromosomes (24 decks)

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