Bioinformatics Flashcards
1
Q
What is resequencing?
A
Mapping a sequence to a reference genome (genome from another individual of same species or closely related species)
2
Q
How is genomics studied?
A
-bio samples sequenced
-de novo assembly to produce consensus sequence or reads assembled by mapping to a reference genome
-tells us structure of genome
3
Q
How is transcriptomics studied?
A
-transcription levels measured
-tells us about gene expression (which genes expressed and when and by how much)
4
Q
What are the methods of studying gene expression (transcriptomics)?
A
-Northern blotting
-RT-qPCR
-microarrays
-RNA-seq
5
Q
What is Northern blotting?
A
A technique used to study gene expression by detecting levels of RNA
