CRISPR 2

Question 1

What is CRISPR-Cas9?

Answer 1

A gene-editing technology

Question 1

What does CRISPR stand for in molecular biology?

Answer 1

Clustered Regularly Interspaced Short Palindromic Repeats

Question 2

What is the main function of Cas9 in the CRISPR system when it was discovered?

Answer 2

To create double-stranded breaks in viral DNA

Question 3

How does sgRNA play a role in the CRISPR-Cas9 system?

Answer 3

The Cas9 nuclease is guided to its target DNA by sgRNA

Question 4

How does the CRISPR system create an immune memory in bacteria?

Answer 4

By storing viral spacer fragments in the bacterial genome

Question 5

How does CRISPR-Cas9 locate the target DNA sequence?

Answer 5

It uses RNA molecules complementary to the target sequence

Question 6

What is the significance of the “off-target effects” in CRISPR-Cas9 gene editing?

Answer 6

It describes the unintended alterations to DNA at sites other than the target

Question 7

Which of the following is NOT a potential application of CRISPR-Cas9 technology?

Answer 7

Creating antibiotic resistant bacteria

Question 8

In the process of heat shock transformation in this lab, which of the following is the correct temperature incubation protocol after adding plasmid DNA?

Answer 8

10 min in ice -> 50 sec in 42oC -> 2 min in ice