CRISPR 1

Question 1

What is a plasmid?

Answer 1

A small, extrachromosomal circular DNA molecule within a cell

Question 2

Which of the following is a characteristic feature of plasmids?

Answer 2

They replicate independently within a cell

Question 3

What is the main purpose of the GFP gene in the pGLO plasmid?

Answer 3

To produce green fluorescent protein (GFP) in bacteria

Question 4

What is the function of the ‘bla’ gene in the pGLO plasmid?

Answer 4

Gene that encodes β-lactamase

Question 5

What is the purpose of ‘bla’ gene expression in bacterial transformation?

Answer 5

It provides antibiotic resistance, allowing scientists to select for bacteria with the plasmid by growing cells with antibiotic

Question 6

In genetic engineering, plasmids are commonly used as:

Answer 6

Vectors for DNA transfer and gene expression

Question 7

Which of the following is false about why bacteria are good model systems for studying molecular biology?

Answer 7

Bacteria can pass its new traits on to its offspring and future generations

Question 8

True or False: An E. coli cell transformed with pGLO will appear green (under UV light) when grown with ampicillin but no arabinose.

Answer 8

False

Question 9

true about the growing conditions of cells transformed with the pGLO plasmid?

Answer 9

1. Arabinose regulates the expression of GFP
2. Arabinose binds to araC to turn on the GFP gene
3. Ampicillin selects for cells that have the pGLO plasmid

Question 10

True or False: An E. coli cell transformed with pGLO will appear white (under UV light or not) when grown under ampicillin and arabinose.

Answer 10

false