CRISPR Flashcards
What does CRISPR stand for?
Clustered Regularly Interspaced Short Palindromic Repeats
What is the primary function of CRISPR technology?
To edit DNA with precision
What are the two main components of CRISPR technology?
- CRISPR-associated (Cas) nuclease
- Guide RNA (gRNA)
What role does the CRISPR-associated (Cas) nuclease play?
Acts as the ‘molecular scissors’ to cut DNA at a specific location
What is the function of Guide RNA (gRNA) in CRISPR?
Directs the Cas nuclease to the target DNA location
Why is CRISPR considered important in gene editing?
It allows precise changes to DNA sequences, crucial for understanding and treating genetic diseases
List three potential applications of CRISPR
- Curing genetic diseases
- Developing new therapies
- Improving agriculture
What is CASGEVY™?
A CRISPR/Cas9 gene-edited therapy approved for treating sickle cell disease or transfusion-dependent beta thalassemia
What are some ethical considerations related to CRISPR technology?
- Potential for unintended consequences
- Implications for human germline editing
What is one advantage of CRISPR in disease research?
Rapid Development of Disease Models and Diagnostics
How does CRISPR offer potential for disease prevention and treatment?
By correcting faulty genes
What are two pros of CRISPR technology?
- Simplicity and Low Cost
- Widespread Use in Research
What is a significant con of using CRISPR technology?
Off-Target Effects
True or False: CRISPR technology poses ethical concerns regarding its misuse.
True
What are some risks associated with genetic therapies like CRISPR?
- Certain types of cancer
- Allergic reactions
- Damage to organs or tissues
What is a challenge related to setting up a CRISPR system?
Time and Effort to Set up and Optimize
What are dual-use concerns in the context of CRISPR?
The ability to engineer pathogens or create genetically modified organisms for malicious purposes
