Cornea, Conj, and Eyelid Culturing (M4) Flashcards
How long does it generally take to get the results from culturing?
24-48 hours
What are the indications that require culturing?
- clinical findings insufficient to diagnose
- severe tissue reaction
- infection not responding to initial treatment
What are the semi-solid/solid culture mediums?
- Blood agar
- chocolate agar
- Sabouraud’s agar
- Thayer-Martin agar
What are the liquid mediums for cultures?
Thioglycolate broth
What is the commercially prepared transport container for cultures?
culturette
What color do gram positive stain? 1. Gram negative? 2
- purple
2. red
What are the ways to view fungi and acanthamoeba for staining/viewing?
- calcofluor
2. confocal microscope
What is the non-invasive in-vivo tool to image the ocular surface?
confocal microscope
What can cytology show the doctor?
- microbial morphology
- human cellular response
- epithelial cell composition
What is the procedure for culturing the inferior conjunctival fornix?
- evert lower lid
- roll cotton tip along conj
- avoid lid margins and eyelashes
What are the symbols that should be made on the agar plate (and what do each represent)?
- top left: vertical squiggle (right conj)
- top right: horizontal squiggle (left conj)
- bottom left: letter “R” (right eyelid)
- bottom right: letter “L” (left eyelid)
When using a Kimura platinum spatula to culture what formation should be made on the agar plate?
nine “C’s”
What should be on the label to the lab when sending in a culture?
- patient name
- doctor name
- date of collection
- eye specimen taken from
- meds if used and when discontinued
- indicate which meds to test for resisitance
What are the indications for using cytology?
- central cornea ulcers
- conjunctivitis
- post-operative infection
- allergic or toxic reactions
- degenerative disease
- neoplasm
How are specimens fixed tot he slide for cytology?
- methyl alcohol
- air dry
- flame
What is the term in the cytology sensitivity report that is for organisms that are sensitive to the named antibiotic at normal doses?
susceptible
What is the term in the cytology sensitivity report that is for organisms that are sensitive to the named antibiotic at high doses?
intermediate
What is the term in the cytology sensitivity report that is for organisms that are not sensitive to the named antibiotic?
resistant
Which cultures are available the same day?
scrapes/smears
Which eye should be cultured for any given sample?
both to give a control
What is used to test for the adenovirus?
RPS Adeno Detector
