Cornea, Conj, and Eyelid Culturing (M4)

Question 1

How long does it generally take to get the results from culturing?

Answer 1

24-48 hours

Question 2

What are the indications that require culturing?

Answer 2

1. clinical findings insufficient to diagnose
2. severe tissue reaction
3. infection not responding to initial treatment

Question 3

What are the semi-solid/solid culture mediums?

Answer 3

1. Blood agar
2. chocolate agar
3. Sabouraud’s agar
4. Thayer-Martin agar

Question 4

What are the liquid mediums for cultures?

Answer 4

Thioglycolate broth

Question 5

What is the commercially prepared transport container for cultures?

Answer 5

culturette

Question 6

What color do gram positive stain? 1. Gram negative? 2

Answer 6

1. purple

2. red

Question 7

What are the ways to view fungi and acanthamoeba for staining/viewing?

Answer 7

1. calcofluor

2. confocal microscope

Question 8

What is the non-invasive in-vivo tool to image the ocular surface?

Answer 8

confocal microscope

Question 9

What can cytology show the doctor?

Answer 9

1. microbial morphology
2. human cellular response
3. epithelial cell composition

Question 10

What is the procedure for culturing the inferior conjunctival fornix?

Answer 10

1. evert lower lid
2. roll cotton tip along conj
3. avoid lid margins and eyelashes

Question 11

What are the symbols that should be made on the agar plate (and what do each represent)?

Answer 11

1. top left: vertical squiggle (right conj)
2. top right: horizontal squiggle (left conj)
3. bottom left: letter “R” (right eyelid)
4. bottom right: letter “L” (left eyelid)

Question 12

When using a Kimura platinum spatula to culture what formation should be made on the agar plate?

Answer 12

nine “C’s”

Question 13

What should be on the label to the lab when sending in a culture?

Answer 13

1. patient name
2. doctor name
3. date of collection
4. eye specimen taken from
5. meds if used and when discontinued
6. indicate which meds to test for resisitance

Question 14

What are the indications for using cytology?

Answer 14

1. central cornea ulcers
2. conjunctivitis
3. post-operative infection
4. allergic or toxic reactions
5. degenerative disease
6. neoplasm

Question 15

How are specimens fixed tot he slide for cytology?

Answer 15

1. methyl alcohol
2. air dry
3. flame

Question 16

What is the term in the cytology sensitivity report that is for organisms that are sensitive to the named antibiotic at normal doses?

Answer 16

susceptible

Question 17

What is the term in the cytology sensitivity report that is for organisms that are sensitive to the named antibiotic at high doses?

Answer 17

intermediate

Question 18

What is the term in the cytology sensitivity report that is for organisms that are not sensitive to the named antibiotic?

Answer 18

resistant

Question 19

Which cultures are available the same day?

Answer 19

scrapes/smears

Question 20

Which eye should be cultured for any given sample?

Answer 20

both to give a control

Question 21

What is used to test for the adenovirus?

Answer 21

RPS Adeno Detector