Core Practicals Flashcards
1
Q
DNA gel electrophoresis
Method
A
- cut DNA fragments with R endonucleases
- place in wells of agarose medium with buffering solution
- add known DNA samples and dye
- electric current
- phosphates of DNA move to anode
- uv light – dye fluoresces
2
Q
DNA gel electrophoresis
Outcomes
A
- DNA separates through gel into bands
- lightest strands travel furthest
- compare bands
3
Q
DNA gel electrophoresis
Equipment
A
- restriction enzymes
- DNA sample
- agar gel
- buffer solution
- known DNA fragments
- dye
- electricity
- UV light
