controls and replicants Flashcards
what is an experimental replicate
-purpose is to ensure that any observed effects are consistent and not due to random chance.
-Each repetition, or replicate, is subjected to the same experimental conditions
-helps identify pattern or trends in data
what is a technical replicant
Definition: Technical replicates involve repeating the same experimental conditions with the same biological sample or material.
Purpose: Technical replicates help assess the precision and reliability of the experimental techniques and measurements. They help account for variability introduced by technical factors, such as equipment precision, pipetting errors, and other technical aspects of the experiment.
what is as biological replicant
Definition: Biological replicates involve using multiple, independent samples or organisms exposed to the same experimental conditions.
Purpose: Biological replicates account for inherent biological variability among different samples. They help researchers determine if observed effects are consistent across different instances of the biological material. Biological replicates are particularly important when studying complex living systems, such as cells, tissues, or organisms.
what factors go into choosing the number of replicants
the choice of replicates depends on a combination of statistical considerations, the nature of the biological system, and practical constraints. Researchers aim to strike a balance that ensures reliable and statistically meaningful results within the constraints of available resources.
