control of microbial growth Flashcards
Which of the following is the best way to sterlize the agar growth medium used in lab?
autoclaving
Which chemical agents are sterlizing agents:
Glutaraldehyde
Ethylene oxide
You are tasked to sterlize a mattress contaminated from a patient with Ebola. Which of the follwing methods would you use?
Ethylene oxide
If a object is disinfected does it mean it is sterile? What is it normally used on?
No, not sterile, used on inanimate objects
Can anti-septic be used on skin?
yes
what is sepsis and asepsis
sepsis- bacterial contamination
asepsis- absence of significant contamination (not sterile)
What is sterilization? and what does it target
process in which all forms of microbial life is destroyed. Target clostridium botulinum endospores
static vs cidal
static - inhibits growth
Cidal- kills microorganism (not necessarily the endospores)
what does lower temp (refrigeration) do to bacteria?
decrease rate of growth
Sanitation
reduction of pathogen on food service items
Degerm
removal of transient microbes from skin via mechanical cleaning
Physical agents that sterilize
Moist heat:
-autoclave
-moist sterilization (Ultra-high temp) used to sterilize milk
Dry heat:
-hot-air sterilization(ovens)
Limitations for autoclaving
heat-sensitive materials
what is pasteurization
method developed by Pasteur to destroy microbes in wine and beer
what does uv light do to dna?
Damages DNA–> thymine dimers
