Compre Exam Flashcards
What is the increase in the size of an organ due to an increase in the number of cells?
Hyperplasia
What is the most critical step in tissue processing?
Fixation
What type of necrosis is seen in a patient with pulmonary tuberculosis?
Caseous necrosis
PAS-positive substances are stained what color?
PAS-positive substances are stained what color?
How many changes of wax are required at 15-minute intervals to ensure complete removal of the clearing agent from tissue by manual impregnation?
Four changes
Jelly-like clots in peritoneal, pleural, and pericardial fluids may be prevented by the addition of what substance?
Heparin
What fluids are used for the diagnosis of rabies? (2)
Carnoy’s Fluid and Acetone
What is the recommended concentration of nitric acid, the most common and fastest decalcifying agent?
5-10%
Frozen sections are commonly used for which three purposes?
Rapid pathologic diagnosis during surgery, enzyme histochemistry, and demonstration of lipids
What is added to Mayer’s egg albumin to prevent the growth of molds?
A: Thymol crystals
A tissue exposed to short bursts of carbon dioxide for a few minutes will do what?
A: Freeze
What is the angle formed between the cutting edge of the microtome knife?
A: 27°-32°
What dehydrating agent contains hydrochloric acid?
A: Von Ebner’s Fluid
For the production of polyclonal antibodies, which animal is most frequently used?
A: Rabbit
What is used for the demonstration of neuroglia in frozen sections?
A: Victoria Blue
Which oxidizing agent cannot accelerate the ripening of hematoxylin?
A: Potassium chloride
What is considered to be the most dangerous type of microtome?
A: Standard Sliding Microtome
In the double embedding method, tissues are first infiltrated with what and then embedded in what?
A: Infiltrated with celloidin, then embedded in paraffin
Which clearing agent is recommended for clearing embryos, insects, and very delicate specimens?
A: Aniline Oil
Removal of excessive mercuric fixatives is accomplished by using what?
A: Alcoholic Iodine
Which embedding mold is not dispensable?
A: Plastic Embedding Rings and Base Mold
What is considered to be the most rapid fixative?
A: Carnoy’s Fluid
Which direction is correct for stropping in knife sharpening?
A: Toe to Heel Direction
In the processing of tissues, when is infiltration done?
A: After clearing, before embedding
What should be done if the tissue is soft when the block is trimmed?
A: Repeat the whole procedure
Excessive exposure to which clearing agent may damage the bone marrow, resulting in aplastic anemia?
A: Benzene
What is the smear preparation technique where the material is rapidly and gently applied in a direct or zigzag line throughout the slide?
A: Streaking
What staining method is used to stain living cells immediately after removal from the living body?
A: Supravital Staining
: What is the recommended ratio of fluid to tissue volume for decalcification?
A: 20:1
Which cell found in cervico-vaginal smears presents a honeycomb appearance?
A: Endocervical glandular cells
Who developed the Cambridge microtome?
A: Paldwell Trefall
The refractive index of the mountant should be as near as possible to that of the glass, which is what?
A: 1.518
Which hone is used for badly nicked knives?
A: Fine Carborundum
What can cause xylene to turn milky when tissue is placed in it?
A: Incomplete dehydration
It is both a nuclear and a histochemical fixative
Newcomer’s Fluid
What is the refractive index of Canada Balsam?
1.524
Cellulose adhesive used in ringing:
Durofix
It is the most reliable way of determining the extent of decalcification.
Radiological Method
Glacial acetic acid solidifies at
17°C
Which of the following are microanatomical fixatives?
1. Heidenhain’s Susa 2. Bouin’s 3. Brasil’s 4. 10% NBF 5. Carnoy’s
1,2,3,4
Use of specific dyes which differentiate particular substances by staining them with a color that is
different from that of the stain itself.
Metachromatic staining
Cytoplasmic fixatives:
1. Kelly’s fluid 2. Muller’s Fluid 3. Carnoy’s fluid 4. Bouin’s 5. Orth’s fluid
1,2,5
It refers to the selective removal of excess stain from the tissue in order that a specific substance
may be stained distinctly from the surrounding tissues.
Differentiation
It is an instrument equipped with a warm plate to manage the impregnated specimen, and a cold
plate at -5 deg C for rapid solidification of the block.
Tissue Tek
This is the iron-free brownish-yellow pigment of hemoglobin, found in places of poor oxygenation,
patiricipating in the formation of bile pigment
Hematoidin
Gram-Twort stain for bacteria stains gram-positive organisms:
Blue-black
The most critical step in tissue processing
Fixation
Trichloroacetic acid is
both a fixative and a decalcifying agent
The most common and fastest decalcifying agent is
nitric acid
Recommended clearing agent for embryos, insects and very delicate specimen?
Aniline oil
A knife that is 100 mm in length and is recommended for frozen sections:
Plane-wedge knife
Excellent fixative for glycogen demonstration.
Picric acid fixatives
An intermediate filament that is always positive for melanomas and schwannomas
Vimentin
It is a special way of preserving tissues by quenching and subsequent desiccation of fresh tissues by
sublimation without the use of any chemical fixative
Freeze drying
An autopsy technique in which thoracic, cervical, abdominal and pelvic organs are removed en
masses and subsequently dissected into organ blocks.
M. Letulle