COMPONENT PREPARATION Flashcards
Collecting whole blood and transforming it into components uses what type of method
Centrifugation
a mechanical devices that apply pressure to the blood bag, which allows blood components to flow from one bag to another
Plasma expressor
quality of each scales using calibrated standard weights should be performed periodically to ensure that the scale is performing within specification
scales
the tubing that connect blood bag is usually made of
(PVC) Polyvinyl chloride
it uses a combination of targeted radio frequency energy and pressure to melt and seal the tubing
Tube sealers
allows two separate blood bag to be connected via their PVC tubing without breaching the integrity of either container
Sterile connection device
Uses to freeze plasma, liquid components may be placed in a standard -18 degree C or colder freezer
Plasma freezer
storage devices include:
refrigerators
freezers
platelet agitators
how many anticoagulant -preservative for every 100 mL of whole blood targeted for collection
14mL
whole blood must be stored at what temperature
1-6 C
Quality control indicator for this component are NOT routinely measure
Whole blood
red blood cells is separated from whole blood by what methods
Centrifugation
Apheresis
Sedimentation (less commonly)
AS (additive solution ) must be added to the RBC component within how many days
3 days
RBC WITH additive solution, resulting in a finished product with a hematocrit of
55% to 65%
RBC WITHOUT additive solution must have a finished hematocrit of
65% to 80%
this component must be drawn by a single nontraumatic venipuncture and the concentrate must be prepared within 8 hrs of collection
Platelet
Platelets derived from whole blood
Random Donor Platelet (RDP)
Platelet product obtain from apheresis donation
Single Donor Platelet (SDP)
how to store platelet concentration
Room temp
20-24 C with constant agitation
RDP should contain how many platelets
5.0 x 10^10 platelets
SDP should contain at least how many platelets
3.0 x 10^11 platelets
it is the removal of white blood cell from blood or blood components prior to transfusion
Leukocyte reduction or leukoreduction
SDP should only contain how many residual white blood cells
5.0 x 10^6 residual white blood cells
RDP should only contain how many residual white blood cells
8.3 x 10^3 residual white blood cells
at least how many percent of the original component must be recovered after leukoreduction
85%
it protects against transmission of CMV to patients at increased risk of CMV disease
leukoreduction
frozen within 8 hours
FFP - fresh frozen plasma
frozen within 24 hrs
PF24- plasma frozen within 24 hrs
FFP and PF24 can be stored for 1 years at what temp
-18C
FFP & PF24 can be stored for 7 yrs at what temp
-65 C
thawed temperature
30 - 37 C
FFP can be manufactured into
cryoprecipitate and cryo-poor plasma
What is the volume of FFP or PF24 from apheresis
as much as 800mL
what is the volume of a unit of FFP or PF24 from whole blood
200-375 mL
most often used for transfusion or plasma exchange in patients with thrombotic thrombocytopenia purpura
CPP - cryo-poor plasma
cryoprecipitate pooled in the transfusion service laboratory using an open system must be transfused with how many hours
4hrs
FDA and AABB recommended a minimum dose of gramma irradiation of ____ to the central portion of the blood unit
25Y
irradiation may be achieved by using what radioactive sources
cesium-137
cobalt-60
x-ray
it is often transfused during the neonatal period or in infants younger than 4 months of age due to their small blood volume
aliquoted blood products
it allows multiple blood components to be transfused at a single event
pooled components
T/F
multiple units of red blood cells are NOT typically pooled together, but ither product types may be pooled
TRUE
platelets pooled in an open systems must be transfused within how many hrs
4rhs of pooling
it is stored for up to 10 yrs for those patients with rare phenotypes, for autologous use
frozen, deglycerolized RBCs
-most widely used
-increases the cryoprotective power of the glycerol, thus allowing slow, uncontrolled freezing process
-provides storage at -65 C or below
high glycerol (40% weight per volume)
Cryoprotection of the glycerol in minimal, and a very rapid, more controlled freezing procedure is require
-liquid nitrogen is routinely used for this method
Low glycerol (20% weight per volume)
used to treat patients with hemophilia A and have almost completely replaced cryoprecipitate as the product of choice
Factor VIII concentrates
